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Addgene

We narrowed to 11,735 results for: pcDNA

Showing: 10051 - 10100 of 11735 results
  1. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Insert
    cGR2176
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pDNA3-Cerulean-FHA1-NES

    Plasmid
    #138202
    Purpose
    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.
    Depositor
    Insert
    Cerulean-FHA1-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid
    #137827
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Insert
    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. FRB_nPPVp

    Plasmid
    #118966
    Purpose
    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain
    Depositor
    Insert
    split nPPVp in fusion with FRB
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pSSA-PMG (pXK-1649)

    Plasmid
    #208857
    Purpose
    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. Bongwoori

    Plasmid
    #63720
    Purpose
    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D
    Depositor
    Insert
    Bongwoori
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. cycLuc_SuMMVs

    Plasmid
    #119210
    Purpose
    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Insert
    cyclic luciferase with SuMMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pSYN-VWF-039

    Plasmid
    #120292
    Purpose
    VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein
    Depositor
    Insert
    VWF D1-D2-D'-D3-Fc
    Use
    Tags
    Fc
    Expression
    Mammalian
    Mutation
    C1099A/C1142A
    Promoter
    CMV
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. cycLuc_SbMVs

    Plasmid
    #119209
    Purpose
    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Insert
    cyclic luciferase with SbMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pTS2311-Tier1-spacer-PPGK-NLuc_TB

    Plasmid
    #169552
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Insert
    PPGK-NanoLuc Luciferase - mTagBFP fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 10051 - 10100 of 11735 results