We narrowed to 7,271 results for: Tet-on

1. pET-28c S2

   Plasmid

   #171920

   Purpose

   Express RNA-based (p)ppGpp sensor

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Insert

   RNA-based (p)ppGpp sensor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPLV_C1

   Plasmid

   #186415

   Purpose

   Endogenous murine RNase inhibitor production for cell-free lysate

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

   Insert

   Murine RNase Inhibitor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

   Plasmid

   #217725

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE4
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-179

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTurquoise2-ER

   Plasmid

   #36204

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   ER-mTurquoise2
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHis17_ParM _2

   Plasmid

   #78202

   Purpose

   Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.

   Insert

   parM (K33A D63C T174A T175N D224C C287A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A D63C T174A T175N D224C C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pComb3HTT

   Plasmid

   #63900

   Purpose

   Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Use

   Phage display

   Tags

   geneIII

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM1s3TsR

   Plasmid

   #137922

   Purpose

   E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

   Plasmid

   #211466

   Purpose

   Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   mito-mCherry-GFP1-10
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAraH6HATT

   Plasmid

   #63901

   Purpose

   For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Tags

   6xHis and HA tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-PDZD8HA

    Plasmid

    #105005

    Purpose

    Mammalian Expression of PDZD8

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    PDZD8 (Pdzd8 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-3C-sGFP11_pcDNA3.1(-)

    Plasmid

    #182932

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 11, FLAG tag, and HA signa…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-3C-sGFP1-10_pcDNA3.1(-)

    Plasmid

    #182931

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Tags

    3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmRFP_a_tubulin_C1

    Plasmid

    #21040

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    pmRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEVA221TadA*-T7RNAP

    Plasmid

    #167980

    Purpose

    Expresses fusion protein TadA*-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    TadA*-T7RNAP
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFU-AgaIVA-VG

    Plasmid

    #24145

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

    Insert

    Venus-AgatoxinIVA-FLAG-GPI anchor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSECRETS-A

    Plasmid

    #196986

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pltetO1

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Inducible tdTomato

    Plasmid

    #183718

    Purpose

    tdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Inserts

    tdTomato
    rTetR
    

    Use

    Lentiviral

    Promoter

    TRE3GV and UbiC

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCL66-decoder

    Plasmid

    #159453

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    Pfus1mut

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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