We narrowed to 35,304 results for: lat

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pCAGGS_cAMPFIRE-L

Plasmid

#182283

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_3XHA-ATP6V1B2-mNeonGreen

Plasmid

#228865

Purpose

Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V1B2 (ATP6V1B2 Human)

Use

Lentiviral

Tags

3xHA and mNeonGreen

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)

Plasmid

#119826

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCDH_TNF-SBP-EGFP

Plasmid

#65282

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

Insert

PQBP1 (PQBP1 Human)

Tags

6xHA

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1-EPSIN 2

Plasmid

#22242

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad3 (3A)

Plasmid

#14964

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (3A) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

Three Ser to Ala mutations in the phosphorylation…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pRSII424

Plasmid

#35466

Depositor

Steven Haase

Article

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast 2 micron vector

Promoter

None

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSIMcpf1

Plasmid

#153034

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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TVT7R(0,0)

Plasmid

#98631

Purpose

Vector for cloning infectious RNA virus genomes

Depositor

Andrew Ball, Karyn Johnson

Article

Johnson et al J Virol. 2000 Jun;74(11):5123-32.

Type

Empty backbone

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT234

Plasmid

#127855

Purpose

Repressilator 2.0, without degradation tags, integrated triple reporter

Depositor

Johan Paulsson

Article

Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

Inserts

bla
mSCFP3
mKate2
mVenus
TetR
LacI
cI

Use

Synthetic Biology

Expression

Bacterial

Mutation

First 11aa derived from mCherry as fusion protein

Promoter

pAmpR, pL LacO1, pL tetO1, and pR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-T/O-Ds1-mCherry

Plasmid

#112855

Purpose

Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

Depositor

David Sprinzak

Article

Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

Insert

DCHS1 (DCHS1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

doxycycline inducible promoter

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG358

Plasmid

#115599

Purpose

surface-displayed Ag1; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pDW465

Plasmid

#8846

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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