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pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Publication

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDP-43

Plasmid

#235487

Purpose

Expression of human TDP-43-EGFP

Depositor

Maxime Rousseaux

Publication

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TARDBP (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5 HA-GST-Presc-mAkt1 (wt)

Plasmid

#48805

Purpose

expresses wt Akt1

Depositor

David Sabatini

Publication

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-MAGI1

Plasmid

#23523

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAGI1 (MAGI1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
TFORF2538

Plasmid

#144036

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX4 (SOX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0985

Plasmid

#142249

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mNG

Plasmid

#183460

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Publication

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Erika Holzbaur, Andrea Stavoe

Publication

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Publication

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Publication

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jag1 (Jag1 Rat)

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC151-3XUAS-IVS-Flp2::PEST

Plasmid

#32133

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Flippase2::PEST

Tags

PEST

Expression

Insect

Mutation

Glycine at Amino Acid 5

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCG

Plasmid

#51476

Purpose

mammalian cell expression vector

Depositor

Winship Herr

Publication

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Type

Empty backbone

Expression

Mammalian

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pUG2K

Plasmid

#35493

Depositor

Ramesh Shivdasani

Publication

Horst et al Cancer Res. 2012 Feb 8.

Insert

KRAS(G12V) (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V

Promoter

Ubiquitin C

Available since

May 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSC101-AmpR EV

Plasmid

#208184

Purpose

empty plasmid vector, non-fluorescent

Depositor

Tanel Tenson

Publication

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Type

Empty backbone

Use

Reporter

Expression

Bacterial

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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AdML

Plasmid

#11242

Depositor

Robin Reed

Publication

Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

AdML

Expression

Bacterial

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-CaMKII-Chrimson-GFP

Plasmid

#62718

Purpose

AAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-myc3-14-3-3 Beta

Plasmid

#19957

Depositor

Yue Xiong

Publication

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

14-3-3 Protein Beta (YWHAB Human)

Tags

Myc3

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles