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pAM1573

Plasmid

#40239

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

Cm Resistance, neutral site II integration platform for Synechococcus

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-mCherry-WT-KRAS-IRES-Blast

Plasmid

#153335

Purpose

Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells

Depositor

Josef Penninger

Publication

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

mCherry-WT-KRAS (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF2

Plasmid

#141189

Purpose

Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Publication

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF2 (GIGYF2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-puro-N-3HA-SREBP2-C-Flag

Plasmid

#134287

Purpose

Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP2

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

3x HA and Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Publication

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.Flag.HERC2-HECT-WT.6xHis

Plasmid

#39227

Depositor

Aziz Sancar

Publication

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

T7

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pTG-mTDG

Plasmid

#52266

Purpose

Bacterial expression of mouse TDG with C-terminal GST-tag

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI

Plasmid

#50723

Purpose

overexpression of human TPI1 in E.coli

Depositor

Markus Ralser

Publication

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRK5-mFzd1

Plasmid

#42253

Depositor

Chris Garcia, Jeremy Nathans

Publication

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 1 (Fzd1 Mouse)

Expression

Mammalian

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
human K19

Plasmid

#14744

Depositor

M Bishr Omary

Publication

Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

Insert

Keratin 19 (KRT19 Human)

Expression

Mammalian

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Publication

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Tags

HA

Expression

Bacterial

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
Frb-N-TEV

Plasmid

#100095

Purpose

SNIPer plasmid, N terminal TEV fused to Frb

Depositor

Jim Wells

Publication

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

N-TEV

Use

Retroviral

Tags

Frb and Myc

Expression

Mammalian

Mutation

N terminal domain

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Publication

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Promoter

CAG

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Gateway Cloning

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-SGK

Plasmid

#23708

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SGK (SGK1 Human)

Use

Gateway Cloning

Mutation

F430L

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUCmini-iCAP-PHP.AX

Plasmid

#195218

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

Depositor

Viviana Gradinaru

Publication

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

Insert

Synthetic construct isolate AAV-PHP.AX VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFR-FR

Plasmid

#179263

Purpose

Human EGFR C-terminally labeled with FusionRed

Depositor

Jared Toettcher

Publication

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

FusionRed

Promoter

SFFV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

Plasmid

#101063

Purpose

AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

CaMPARI2_F391W-G395D

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMP-YY1_2

Plasmid

#36358

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

YY1 (YY1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles