Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 21,788 results for: his

Showing: 6061 - 6090 of 21788 results

  1. pET28B-Cwc25

    Plasmid
    #111340
    Purpose
    his tagged Cwc25
    Depositor
    Christine Guthrie
    Insert
    CWC25
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111340-plasmid-map-sequence-id-217722

    Enlarge

  5. pGM202T7

    Plasmid
    #69993
    Purpose
    E. coli/Streptomyces expression vector containing the T7-promoter
    Depositor
    Günther Muth
    Article
    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
    Inserts
    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    Use
    Tags
    N-terminal and C-terminal His tag encoding sequen…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69993-plasmid-map-sequence-id-129665

    Enlarge

  7. pJL1-sfGFP-DQNAT

    Plasmid
    #198210
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198210-plasmid-map-sequence-id-393676

    Enlarge

  8. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Article
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60621-plasmid-map-sequence-id-208224

    Enlarge

  12. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Use
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

    Enlarge

  15. pET24a-ncOGT-FL

    Plasmid
    #190821
    Purpose
    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E.coli
    Promoter
    T7
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190821-plasmid-map-sequence-id-379184

    Enlarge

  18. pTDpelB-C_sfYFPTwinStrep

    Plasmid
    #45944
    Purpose
    expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Insert
    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    Use
    Tags
    PelB export sequence and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq/Ptrc
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45944-plasmid-map-sequence-id-64657

    Enlarge

  22. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

    Enlarge

Showing: 6061 - 6090 of 21788 results