We narrowed to 13,291 results for: cDNA

1. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

   Plasmid

   #216494

   Purpose

   Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAktKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-GAlphaoB-RLuc8

   Plasmid

   #140977

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaoB-Rluc8 (GNAO1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4 HisMax-V5-GFP-RRBP1

   Plasmid

   #92150

   Purpose

   Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   RRBP1 (RRBP1 Human)
   

   Use

   Tags

   Calreticulin signal peptide, EGFP (A207K mutation…

   Expression

   Mammalian

   Mutation

   In RRBP1: N951S and T1329L. E1279 is also missing.

   Promoter

   CMV

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

   Plasmid

   #194681

   Purpose

   CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FKBP-EGFP-HOTag3

   Plasmid

   #106924

   Purpose

   Expresses FKBP-EGFP-HOTag3 in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   FKBP-EGFP-HOTag3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cry2 mCherry GRK2 ct in pcDNA3.1

   Plasmid

   #64212

   Purpose

   mCherry fused between Cry2 and GRK2ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK2 ct (GRK2 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and cmv

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1/V5-His Snk/Plk2

   Plasmid

   #16015

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

8. IRE1 alpha-pcDNA3.EGFP

   Plasmid

   #13009

   Depositor

   Fumihiko Urano

   Article

   Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

   Insert

   Inositol Requiring Enzyme-1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FKBP-Venus-173-C

   Plasmid

   #87617

   Purpose

   FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   FKBP-Venus173-C
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP

    Plasmid

    #187073

    Purpose

    Mitochondria surface expression of dHaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-dHaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dHaloTag7 = HaloTag7-D106A

    Promoter

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-TFEBS211A-MYC (#805)

    Plasmid

    #99957

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    6xHIs and Myc

    Expression

    Mammalian

    Mutation

    S211A

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Ras-LOCKR-PL Key

    Plasmid

    #216503

    Purpose

    Ras activity dependent proximity labeler, Key only, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-PL Key
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 Ras-LOCKR-PL Cage

    Plasmid

    #216502

    Purpose

    Ras activity dependent proximity labeler, Cage only, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-PL Cage
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-GFP-Dub3 mouse

    Plasmid

    #72683

    Purpose

    mammalian expression of GFP-Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

    Plasmid

    #169327

    Purpose

    Expression of CEP41-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag7 (CEP41 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP-Cdc42(Q61L)

    Plasmid

    #12600

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (Q61L) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1477 pcDNA3 flag HA Akt1

    Plasmid

    #9021

    Depositor

    William Sellers

    Article

    Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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