173,520 results

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Spectrin-M-cpstFRET

Plasmid

#61109

Purpose

Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

Insert

spectrin-cpstFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pT3EF1aH-myr-Akt

Plasmid

#179909

Purpose

This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

Depositor

Xin Chen

Article

Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

Insert

Akt1 (Akt1 Mouse)

Tags

HA and Myr

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)

Plasmid

#27097

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

VN155(I152L)

Tags

Myc

Expression

Mammalian

Mutation

I152L

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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OKSIM

Plasmid

#24603

Depositor

Jose Cibelli

Article

Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

Insert

Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Oct4-2A-KLF4-2A-Sox2-IRES-Myc

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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ABE8e-Cas9NG

Plasmid

#203328

Purpose

Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

ABE8e-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pemiRFP670-N1

Plasmid

#136556

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-Lepr_FL-IRES-eGFP

Plasmid

#196635

Purpose

Doxycycline inducible expression of full length mouse Lepr with eGFP marker

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

Lepr (Lepr Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-pcDNA3

Plasmid

#18950

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-NE2m

Plasmid

#208686

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-Luc-mCherry-puro

Plasmid

#183685

Purpose

Lentiviral vector expressing luciferase and mCherry.

Depositor

Andrew Kung

Article

Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

Insert

Luc-E2A-mCherry-T2A-PuroR

Use

Lentiviral

Promoter

Human Ubiquitin C (UbC) promoter

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange

Plasmid

#177827

Purpose

Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSIE1

Plasmid

#136355

Purpose

Selection by Inducible Effectors

Depositor

Andrew Goodman

Article

Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

Inserts

Type VI effector bfe1
Type VI effector bte1
BT_4676
tetR

Use

Unspecified

Mutation

ATG removed to add signal sequence before gene

Promoter

P1TDP-GHO23

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Wnk1

Plasmid

#38780

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
ML1Nwt_in_pEGFP-C1

Plasmid

#67797

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV9-CMV-eGFP

Plasmid

#230928

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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