We narrowed to 4,214 results for: Abo;

1. pFLAG-4S Tetherin

   Plasmid

   #41073

   Depositor

   Paul Spearman

   Article

   Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

   Insert

   Tetherin (BST2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S74, S84, 137S, and 144S

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pFLAG-137.144S Tetherin

   Plasmid

   #41072

   Depositor

   Paul Spearman

   Article

   Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

   Insert

   Tetherin (BST2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   137S, 144S

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3-HA/Bmal I317D

   Plasmid

   #47347

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   I317D

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pFLAG-74.84S Tetherin

   Plasmid

   #41071

   Depositor

   Paul Spearman

   Article

   Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

   Insert

   Tetherin (BST2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   74S, 84S

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3-HA/Bmal L115E

   Plasmid

   #47345

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L115E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3-HA/Bmal L95E/L150E/W427A

   Plasmid

   #47352

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E/L150E/W427A

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3-HA/Bmal W427A

   Plasmid

   #47349

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   W427A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3-HA/Bmal V435R

   Plasmid

   #47350

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   V435R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3-HA/Bmal L150E

   Plasmid

   #47346

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L150E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCS2+ 3xFLAG-FEM1B C186S

    Plasmid

    #230995

    Purpose

    Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Cys186 to Ser

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+ 3xFLAG-FEM1B L597A

    Plasmid

    #230994

    Purpose

    Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Leu597 to Ala

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid

    #224787

    Purpose

    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBACH1-1, crBACH1-2, crBACH1-3,
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUt-mNF-Cas13d

    Plasmid

    #224789

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LPUtopia-7_CoV-2

    Plasmid

    #224782

    Purpose

    Landing Pad donor vector containing partial RdRp and N gene sequences from SARS-CoV-2 for fluorescent reporter assay

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    partial RdRp and N gene (N, ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid

    #202090

    Purpose

    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2(R195G, C201A)
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    R195G and C201A abolish catalytic activity

    Promoter

    human Synapsin

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHW539

    Plasmid

    #198811

    Purpose

    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    G310D mutation to abolish the binding of cAMP of …

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

    Plasmid

    #191631

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase (R506S mutation, inactive form)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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