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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1444_ECOR31HamA_purification

Plasmid

#223369

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamA

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamA

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1445_ECOR31HamB_purification

Plasmid

#223370

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamB

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamB

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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ttLbCas12a-intron

Plasmid

#241901

Purpose

ttLbCas12a (LbCas12a-D156R)-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

ttLbCas12a (LbCas12a-D156R)-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-RRV-intron

Plasmid

#241902

Purpose

LbCas12a-RRV-intron Gateway entry plasmid

Depositor

Yiping Qi

Article

Luo et al Plant Physiol. 2025 Nov 26;199(4):kiaf61

Insert

LbCas12a-RRV-intron

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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EML8359_pA3A-CBECas9-ALSpro197

Plasmid

#234353

Purpose

Base editor targets to ALS Pro197 locus

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

ALS

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8552_pEML41003-tufApst4-15

Plasmid

#237865

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufA

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufA

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8553_ pEML41003-tufBpst4-15

Plasmid

#237866

Purpose

A suicide vector for replacing 4th to 15th amino acids of tufB

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

tufB

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-ABE8e-dCas9-BSD

Plasmid

#226116

Purpose

Using promoter ef1a expresses ABE8e-dCas9 protein with Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol 8 (2025). https://doi.org/10

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGR721 - SLD3p-TP-DNAP1.Badboy2

Plasmid

#222013

Purpose

Expresses the Badboy2 TP-DNAP1 variant under control of a weak promoter for reducing p1 copy number

Depositor

Chang Liu

Article

Rix et al Science. 2024 Nov 8;386(6722):eadm9073.

Insert

TP-DNAP1 BadBoy2

Expression

Yeast

Promoter

SLD3p

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1129

Plasmid

#236752

Purpose

BTK Type 2-3 fluorescent protein cassette for golden gate assembly. Improved version of pBTK1055

Depositor

Jeffrey Barrick

Article

Lariviere et al mBio. 2024 Sep 11;15(9):e0139224. doi: 1

Insert

Type 2-3 GFP cassette

Use

Synthetic Biology

Promoter

CP25

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL678

Plasmid

#207511

Purpose

GmUbi3-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmUBI3

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL679

Plasmid

#207512

Purpose

GmM4-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL681

Plasmid

#207514

Purpose

GmM4-SpRY ABE-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF3

Plasmid

#232643

Purpose

transient expresses IRF3 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF3 (IRF3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP

Plasmid

#226861

Purpose

Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by Y93H mutation, that can be reactivated by active cytosine base editing

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

EGFP

Expression

Mammalian

Mutation

Y93H

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCBE-dGFP-gRNA

Plasmid

#226862

Purpose

Human gRNA expression vector targeting the Y93H mutation of non-fluorescent EGFP in plasmid pCBE-dGFP; for use with CBEs

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TC alphaDdCBE T2

Plasmid

#221991

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TC TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND2 alphaDdCBE T2

Plasmid

#221977

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ND2 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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