We narrowed to 13,509 results for: GAN

1. pDIRECT_21D

   Plasmid

   #91131

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_NR5A2_Hormone-recep

   Plasmid

   #109818

   Purpose

   Protein expression and purification of NR5A2_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   NR5A2_Hormone-recep (NR5A2 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-shAUF1 hairpin 1

   Plasmid

   #52921

   Purpose

   expresses a hairpin specific for human AUF1

   Depositor

   Sheila Stewart

   Article

   Alspach et al Cancer Discov. 2014 Mar 26.

   Insert

   shRNA targeting AUF1 (HNRNPD Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Jag2ICD-HA pBacPAK8-3

   Plasmid

   #17332

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged2 (Jag2 Rat)
   

   Tags

   HA

   Expression

   Insect

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pGEX 4T1 ERbeta(1-255)

   Plasmid

   #35564

   Depositor

   Harish Srinivas

   Article

   Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

   Insert

   Estrogen Receptor beta (ESR2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   residues 1-255

   Available Since

   March 26, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pZLCv2-gD4Z4-1-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106352

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2519

   Plasmid

   #91076

   Purpose

   Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU3

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_EPB41L3_FERM

   Plasmid

   #110011

   Purpose

   Protein expression and purification of EPB41L3_FERM

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   EPB41L3_FERM (EPB41L3 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMM1157

    Plasmid

    #112534

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    luxCDABE
    

    Expression

    Bacterial

    Promoter

    PL(HrtO) and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM-AAV-hSncg-EGFP

    Plasmid

    #153198

    Purpose

    Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PhyB-mCherry-MYO1

    Plasmid

    #51570

    Purpose

    Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    MYO1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_NR1I2_Hormone-recep

    Plasmid

    #110020

    Purpose

    Protein expression and purification of NR1I2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1I2_Hormone-recep (NR1I2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO-shAUF1 hairpin 2

    Plasmid

    #52922

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCDB181

    Plasmid

    #80692

    Purpose

    for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

    Depositor

    David Baker

    Article

    Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

    Insert

    gEHEE_06
    

    Tags

    OsmY-10His-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJG488

    Plasmid

    #91196

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi8+gUbi1+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. STIMATE(1-240)

    Plasmid

    #71280

    Purpose

    Expresses STIMATE or TMEM110 (1-240) in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

    Insert

    STIMATE (1-240) (TMEM110 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106355

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    MYOD1 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Jag1ICD-HA pBacPAK8-3

    Plasmid

    #17331

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Jagged1 (Jag1 Rat)
    

    Tags

    HA

    Expression

    Insect

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pJL3

    Plasmid

    #184131

    Purpose

    For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

    Depositor

    Alejandro Aballay

    Article

    Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

    Insert

    MRP-1 (mrp-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    Pges-1 (3.3kb)

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lyn-CFP-GAI(1-172)

    Plasmid

    #37313

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-172

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

22. WNT11-bio-His

    Plasmid

    #53430

    Purpose

    Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    WNT11 (WNT11 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFastBac1-OSF-TRIM5alpha(human)(R332P)

    Plasmid

    #79037

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human R332P) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Arg 332 to Pro

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. bactin:mKate2-ras

    Plasmid

    #105963

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    Ras (KRAS Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

    Plasmid

    #127363

    Purpose

    Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lyn-CFP-GAI(1-532)

    Plasmid

    #37314

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-532

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

27. AtS/MAR10 insulator sequence (GB3458)

    Plasmid

    #160638

    Purpose

    pEGB3a1 AtS/MAR10

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    AtS/MAR10 insulator sequence
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSR06

    Plasmid

    #69152

    Purpose

    read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0 and codon-optimzed index…

    Promoter

    rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHH0103_HLA-DRB1_MHC-II-beta

    Plasmid

    #110021

    Purpose

    Protein expression and purification of HLA-DRB1_MHC-II-beta

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    HLA-DRB1_MHC-II-beta (HLA-DRB1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

    Plasmid

    #37606

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP III C840A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C840A

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pY036_ATP1A1_G5

    Plasmid

    #86618

    Purpose

    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid

    #182354

    Purpose

    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid

    #182356

    Purpose

    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEMS2131

    Plasmid

    #111895

    Purpose

    ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_GNA13_G-alpha

    Plasmid

    #109913

    Purpose

    Protein expression and purification of GNA13_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA13_G-alpha (GNA13 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMOD_C2519

    Plasmid

    #91088

    Purpose

    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    OsU3

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lyn-CFP-GAI(1-151)

    Plasmid

    #37312

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-151

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pCRII-Sema4a-ISH-2kb

    Plasmid

    #68029

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4A (Sema4a Mouse)
    

    Use

    Mrna in situ hybridization probe

    Mutation

    a 2kb cDNA fragment for ISH probe synthesis

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GST-AR-1-173

    Plasmid

    #104193

    Purpose

    Expresses human AR aa 1-173 in pGEX-5x-1 bacterial expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-173 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge