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eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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Ubiquitin K63C

Plasmid

#86589

Purpose

Bacterial expression of Ubiquitin K63C for cross linking studies

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C

Promoter

Tac

Available Since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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p4028 GST-E6AP

Plasmid

#8655

Depositor

Peter Howley

Article

Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(

Insert

E6-AP (UBE3A Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR2)

Plasmid

#192811

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR2

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA3

Plasmid

#1784

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

April 28, 2005

Availability

Academic Institutions and Nonprofits only

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XLone-Cas9-2A-GFP

Plasmid

#170159

Purpose

Express Cas9 and GFP upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

Cas9

Use

Synthetic Biology; Piggybac

Tags

GFP

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-UBC9-K14R-A129K

Plasmid

#228948

Purpose

express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

Depositor

Marcin Suskiewicz

Insert

UBC9 (UBE2I Human)

Tags

His tag and thrombine cleavage site : MGHHHHHHSSG…

Expression

Bacterial

Mutation

changed Lysine 14 to Arginine, changed Alanine 12…

Promoter

T7-lac

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dN)

Plasmid

#192808

Purpose

Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dM)

Plasmid

#192810

Purpose

Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the Middle domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dIDR1)

Plasmid

#192809

Purpose

Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the IDR1

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (dC)

Plasmid

#192812

Purpose

Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deletion of the C-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pc3xTC20-alphaTubulin

Plasmid

#36327

Depositor

Amy Palmer

Article

Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

Insert

alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling (TUBA1B Human, Synthetic)

Tags

3xTC20

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2012

Availability

Academic Institutions and Nonprofits only

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p-G4PID

Plasmid

#209638

Purpose

Expression G4PID in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCB004

Plasmid

#134147

Purpose

Bacterial expression of human Ska3

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

human Ska3 (SKA3 Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4 (L63R)

Plasmid

#192814

Purpose

Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

L63R mutation in the N-terminal domain

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAID 1.1C-T2A-Bsr

Plasmid

#105986

Purpose

To make an auxin inducible degron mutant

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

Insert

T2A-Bsr

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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