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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,322 results for: BASE

Showing: 4081 - 4100 of 12322 results

  3. pVC

    Plasmid
    #175063
    Purpose
    expresses mVenus and mCherry pair in mammalian cells
    Depositor
    Arnold Boersma
    Article
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    mVenus + mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175063-plasmid-map-sequence-id-353801

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  7. pCSIIhyg-rtTA

    Plasmid
    #139480
    Purpose
    Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Insert
    rtTA, Tet-ON reverse transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a promoter
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139480-plasmid-map-sequence-id-276014

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  12. P[acman]-Alpha2

    Plasmid
    #165859
    Purpose
    Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening
    Depositor
    Koen Venken
    Article
    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    165859-plasmid-map-sequence-id-336455

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  13. AEB-No-aptamer-controlmRFP1

    Plasmid
    #63848
    Purpose
    This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    J23100
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63848-plasmid-map-sequence-id-106571

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  15. pTEV5_ RpMatB_2

    Plasmid
    #78206
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
    Depositor
    Martin Webb
    Article
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A S170A R286C Q457C K488A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A S170A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78206-plasmid-map-sequence-id-146045

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  17. qTAG-AAVS1-Ef1a-Zeo

    Plasmid
    #227268
    Purpose
    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227268-plasmid-map-sequence-id-451274

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  18. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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Showing: 4081 - 4100 of 12322 results