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We narrowed to 10,084 results for: Coli

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Showing: 4081 - 4100 of 10084 results

  2. pSLS.491

    Plasmid
    #184956
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C
    Promoter
    T7/lac
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184956-plasmid-map-sequence-id-376557

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  3. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  6. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  7. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

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  8. pTA-Mob 2.0

    Plasmid
    #149662
    Purpose
    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.
    Depositor
    Bogumil Karas
    Article
    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:
    Type
    Empty backbone
    Use
    Synthetic Biology; Conjugation helper plasmid
    Expression
    Bacterial
    Available Since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    149662-plasmid-map-sequence-id-379005

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  9. pS3

    Plasmid
    #50596
    Purpose
    Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5
    Depositor
    Jay Keasling
    Article
    Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8
    Inserts
    aroE
    aroD
    aroB
    aroG*
    ppsA
    tktA
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    50596-plasmid-map-sequence-id-77162

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  13. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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  15. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

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  17. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138587-plasmid-map-sequence-id-274478

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Showing: 4081 - 4100 of 10084 results