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pEF6 VPS52-V5-6His

Plasmid

#175096

Purpose

V5 tagged VPS52 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

VPS52 (VPS52 Human)

Tags

V5

Expression

Mammalian

Mutation

N666D- please see depositor comment

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo VPS54-13Myc

Plasmid

#175095

Purpose

13Myc tagged VPS54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

Insert

VPS54 (VPS54 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABE8eHD-BlastR

Plasmid

#176478

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABE8eHD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux

Plasmid

#176239

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp7-EGFP

Plasmid

#165111

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp10-mCherry

Plasmid

#165135

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-NpHR3.3-EYFP

Plasmid

#137151

Purpose

Viral expression of NpHR3.3-EYFP

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

NpHR3.3-EYFP

Use

AAV

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF10-2xCSTREP

Plasmid

#136174

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF10

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF45-2xCSTREP

Plasmid

#136206

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF45

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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FEV_pet28a

Plasmid

#131668

Purpose

Bacterial expression of ETS gene FEV

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FEV (FEV Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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ETV5_pet28a

Plasmid

#131639

Purpose

Bacterial expression of ETS gene ETV5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV5 (ETV5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHIE046 ZF43x12-C mKate2 (TUPV1)

Plasmid

#138726

Purpose

mMoClo TUPV, with ZF43x12-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3xHA-eGFP-OMP25

Plasmid

#239249

Purpose

3xHA-eGFP-OMP25 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

3xHA-eGFP-OMP25 (SYNJ2BP Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pSSRB274_pAC8-eGFP-hsCRBN

Plasmid

#218791

Purpose

Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Tags

FLAG-TEV-eGFP-Prescission

Expression

Insect

Mutation

eGFP fused to N-terminus

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM1b

Plasmid

#211494

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

Depositor

Yasunori Saheki

Article

Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-mCherry-Hygro

Plasmid

#210852

Purpose

Lentiviral expression vector encoding FIP200-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN

Plasmid

#180647

Purpose

Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, has siRNA resistance to GAACAGUGUG…

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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