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Plasmid#120059PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3DepositorInsertDronpa3
UsePcr templateTagsDronpa3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 HA-Amida
Plasmid#19794DepositorInsertAmida (TFPT Human)
UseTags2XHAExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z14
Plasmid#120057PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5DepositorInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK CHAC1 3'UTR
Plasmid#19861DepositorInsertCHAC1 3'UTR (CHAC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1358 - Intron GG1 - smu-1
Plasmid#159804PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFSW Syt1-C2B IRES GFP
Plasmid#133817PurposeEncodes the C2B domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-eGFP
Plasmid#136098PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_eGFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-mTurquoise2
Plasmid#136100PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_mTurquoise2
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS_GUS
Plasmid#136084PurposeFor histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.DepositorInsertCDS_GUS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS12_eGFP-linker
Plasmid#136086PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_eGFP(noStop)-SerGly10xAlaSer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mScarletI-linker
Plasmid#136088PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_mScarletI(noStop)-SerGly10xAlaSer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-P04
Plasmid#120015PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup35-EcoRI
Plasmid#87332PurposeTo express shRNA against human Nup35DepositorInsertshRNA against human Nup35
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2214 - Intron GG1 - 900 bp
Plasmid#159880PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only