We narrowed to 27,048 results for: STI

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmyo-3

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFUS_A

Plasmid

#31028

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Adamts2.1-DIO-GCaMP8s-WPRE

Plasmid

#211491

Purpose

conditional expression of GCaMP8s under artificial promoter Adamts2

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Baz1a.1-DIO-GCaMP8s-WPRE

Plasmid

#211493

Purpose

conditional expression of GCaMP8s under artificial promoter Baz1a

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

DIO-GCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔ+/-

Plasmid

#35378

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 575-611

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pKS011

Plasmid

#65464

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

MBP-Ntag-mCherry

Tags

FLAG and MBP

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJK438

Plasmid

#71590

Purpose

Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

Depositor

T. J. Kappock

Article

Murphy et al Acta Crystallogr F Struct Biol Commun. 2

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJB028

Plasmid

#65469

Purpose

pTrc plasmid expressing p14*-TEV

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

p14*-TEV (TEVgp1 )

Use

Synthetic Biology

Expression

Bacterial

Promoter

trc

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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JGE301_pAAV-hSyn-DIO-Flag-hM3Dq-V2(2D)-ABIcs-mCherry

Plasmid

#107246

Purpose

hM3DREADD fused to ABIcs for abscisic acid mediated recruitment of PYLcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

hM3DREADD

Use

AAV

Tags

ABIcs, FLAG, and mCherry

Mutation

T to D and S to D mutations in V2-tail

Promoter

hSYN

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Six1 (CT#664)

Plasmid

#13876

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Six1 (Six1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCS2-USP44-HIS

Plasmid

#43894

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS

Expression

Mammalian

Available Since

April 12, 2013

Availability

Academic Institutions and Nonprofits only
FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-PTEN-K289E

Plasmid

#30380

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-K289E (PTEN Human)

Expression

Mammalian

Mutation

K289E

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-C124S

Plasmid

#30376

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-C124S (PTEN Human)

Expression

Mammalian

Mutation

C124S

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pTAPmyc-T2A-tagRFP

Plasmid

#140278

Purpose

Backbone for making a mammalian expression vector of the protein of interest

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Empty backbone

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRL8

Plasmid

#24980

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔPQ

Plasmid

#35187

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 34-113

Promoter

CMV

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only
pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only