We narrowed to 17,703 results for: She

1. pBbB6k-GFP

   Plasmid

   #35357

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   LlacO-1

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. RHPMP133 (mOrange2-beta Actin)

   Plasmid

   #88837

   Purpose

   Localization by Super-Resolution microscopy of beta-Actin fused to mOrange2

   Depositor

   Ricardo Henriques

   Insert

   beta-Actin (ACTB Human)
   

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-Flag-Mm Ezh2 Y641F

   Plasmid

   #80077

   Purpose

   Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

   Depositor

   Phillip Abbosh

   Inserts

   Flag-EZH2 Y641F (Ezh2 Mouse)
   Hygromycin phosphotransferase
   

   Use

   Retroviral

   Tags

   5' FLAG

   Expression

   Mammalian

   Mutation

   Y641F, R342R, N393N

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

   Plasmid

   #225433

   Purpose

   Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

   Depositor

   Diego Orzaez

   Insert

   4xCBS:mDFR:Rep/RepA:Tnos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-DestFLAGHA_HuB

   Plasmid

   #65758

   Purpose

   constitutively expresses FLAG-HA-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

6. GPX3

   Plasmid

   #38899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GPX3 (GPX3 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pFRT-TODestGFP_HuB

   Plasmid

   #65761

   Purpose

   expresses GFP-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNC-Green-LUC

   Plasmid

   #193438

   Purpose

   Nimble cloning-compatible vector for the analysis of promoter activity in plants

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-UbC-eGFP-F

   Plasmid

   #71545

   Purpose

   Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

   Depositor

   Pantelis Tsoulfas

   Insert

   eGFP-F
   

   Use

   AAV

   Tags

   contains 20- amino-acid farnesylation signal from…

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSADdeltaG-GFP-mCherry

    Plasmid

    #32643

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    mCherry
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

11. p6VP1

    Plasmid

    #24725

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 6
    

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7VP1

    Plasmid

    #24726

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 7
    

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT-Hygro hTERT FL-Firefly

    Plasmid

    #171057

    Purpose

    hTERT Full-length-expressing splicing minigene

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    hTERT FL-Firefly
    

    Expression

    Mammalian

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV--Mouse Mdm2 wt-IRES-CFP

    Plasmid

    #180493

    Purpose

    Role of Mdm2 splicing variants in tumorigenesis

    Depositor

    Martine Roussel

    Article

    Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

    Insert

    Mdm2 full length (wt)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNIC28-hKdm2b-CxxC-PHD

    Plasmid

    #49216

    Purpose

    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

    Depositor

    Rob Klose

    Insert

    Kdm2b (KDM2B Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hs.NRAS Q61L

    Plasmid

    #83178

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBbS2k-RFP

    Plasmid

    #35330

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTXB1-CII

    Plasmid

    #51566

    Purpose

    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    cII
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBbE8c-RFP

    Plasmid

    #35269

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEX307-APN-G418

    Plasmid

    #158456

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBbS1c-RFP

    Plasmid

    #35338

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Trc

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBbE6c-RFP

    Plasmid

    #35287

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid

    #78336

    Purpose

    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    PAX3 (PAX3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

30. Hs.HRAS Q61R

    Plasmid

    #83188

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbB5a-GFP

    Plasmid

    #35352

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUG-amdSYM-mak10TR

    Plasmid

    #75433

    Purpose

    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

    Depositor

    John McCusker

    Inserts

    MAK10TR
    ApR
    amdSYM
    

    Expression

    Yeast

    Mutation

    N-terminal 740 bp of MAK10 ORF

    Promoter

    Native promoter and PTEF

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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33. Hs.HRAS G12D

    Plasmid

    #83183

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12D

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Hs.NRAS Q61K

    Plasmid

    #83180

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61K

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NEK2

    Plasmid

    #39163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NEK2 (NEK2 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    T175A inactivating mutant

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pDONR221-CTSB

    Plasmid

    #158443

    Purpose

    Gateway Cloning compatible entry vector for the human CTSB gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSB (CTSB Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSA101

    Plasmid

    #59787

    Purpose

    drives ZIF-1 protein in C. elegans neurons

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    rab-3 promoter
    zif-1 coding (zif-1 Nematode)
    unc-54 3' UTR
    

    Available Since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 4H4H

    Plasmid

    #51314

    Depositor

    Cheryl Arrowsmith

    Insert

    4H4H (EHMT1 Human, 4H4H)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

39. pAAV-DIO-EF1a-TVA-P2A-EYFP

    Plasmid

    #177017

    Purpose

    For expression of the avian TVA receptor in a cre-dependent manner

    Depositor

    Peter Jonas

    Insert

    TVA-P2A-EYFP (CD320 Coturnix japonica)
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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40. pLenti-cytoMetGFP

    Plasmid

    #37561

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    only contains cytoplasmic domain amino acids K956…

    Promoter

    CMV

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge