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Syntaxin-4 M1 (Y115A)

Plasmid

#44029

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM-BAF180-SH3

Plasmid

#41933

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM-BAF180-SH1

Plasmid

#41931

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
sugarless cDNA

Plasmid

#42063

Depositor

Norbert Perrimon

Article

Hacker et al Development. 1997 Sep;124(18):3565-73.

Insert

sugarless (sgl Fly)

Available Since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
pFM114

Plasmid

#40160

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N+S251A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa 1-364); S251A

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM116

Plasmid

#40162

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C+S950A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa840-1268); S950A

Promoter

tac

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 923 to 1179

Plasmid

#23995

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 923 to 1179

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 1 to 177

Plasmid

#23993

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA 5'ETS ntds 1 to 177

Use

Bacterial cloning - topo

Mutation

None

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-25S rDNA

Plasmid

#23996

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

25S rRNA from +5270 to oligo y

Use

Bacterial cloning - topo

Mutation

none

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pGMEH-P38-luxAFB-DAS+4

Plasmid

#27064

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxA-FLAG-luxB-DAS+4

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB-DAS+4

Plasmid

#27067

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-DAS+4 integrative

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pPS834

Plasmid

#8932

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Tags

myc

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
C077

Plasmid

#174346

Purpose

Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Availability

Academic Institutions and Nonprofits only

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C078

Plasmid

#174347

Purpose

Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

In vitro transcription template

Tags

FLAG

Mutation

13 silent mutations: G243A, A273G, G339A, C345G, …

Availability

Academic Institutions and Nonprofits only

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C233

Plasmid

#174349

Purpose

Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (M161A) (pabpc1.S Frog)

Use

In vitro transcription template

Tags

FLAG

Mutation

M161A

Availability

Academic Institutions and Nonprofits only

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pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-Blimp1

Plasmid

#73995

Purpose

Plasmid expressing mouse Blimp1

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Blimp1 gene (Prdm1 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro

Plasmid

#85132

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Hygro

Plasmid

#85134

Purpose

lentiviral backbone

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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