4,180 results

1. MSP2133

   Plasmid

   #72249

   Purpose

   Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y450A, N497A, R661A, Q695A, and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTSA-13

   Plasmid

   #17327

   Depositor

   Takeshi Sano

   Article

   Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

   Insert

   streptavidin
   

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Available Since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR/pSUPER+ (575-1)

   Plasmid

   #17338

   Purpose

   Gateway entry vector for shRNA expression under H1 promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   RNAi; Entry vector

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. piRFP702-N1

   Plasmid

   #45456

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP702
   

   Expression

   Mammalian

   Mutation

   T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 mouse src K295R Y527F

   Plasmid

   #13657

   Depositor

   Joan Brugge, Peter Howley

   Insert

   src K295R Y527F (Src Mouse)
   

   Expression

   Mammalian

   Mutation

   K295R Y527F. Dominant negative mutant.

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP

   Plasmid

   #35499

   Purpose

   AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Has Service

   AAV9

   Insert

   ChR1-VChR1 Chimera
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E122T and E162T

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr

   Plasmid

   #19326

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Pmyo-3::twk-18(cn110)cDNA
   

   Expression

   Worm

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-RhoA FLARE.sc Biosensor T19N

   Plasmid

   #12152

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor T19N (RHOA Human)
   

   Tags

   6xHis

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   RhoA T19N (dominant-negative mutation)*

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CaMPARI V398D

    Plasmid

    #60786

    Purpose

    Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI V398D
    

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    V398D

    Promoter

    CMV

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-neo largeTgenomic

    Plasmid

    #10891

    Depositor

    William Hahn

    Insert

    large T and small T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

12. LV-Lac

    Plasmid

    #12108

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2001 Sep 25. 9

    Insert

    LacZ
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pSV Sport PPAR gamma 2

    Plasmid

    #8862

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSV Sport PPAR gamma 1

    Plasmid

    #8886

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

19. siSlug3

    Plasmid

    #10905

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    siSlug (SNAI2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. shp63alpha pLKO.1 puro

    Plasmid

    #19120

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p63 (TP63 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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