We narrowed to 6,927 results for: 11

1. cytosolic moPrP (3F4)

   Plasmid

   #1321

   Depositor

   Susan Lindquist

   Article

   Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

   Insert

   PrP (Prnp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-term truncation (to make gene cytosolic), vario…

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

2. Cry2PHR.mCh.CofS3E

   Plasmid

   #133619

   Purpose

   Expresses Cry2PHR - mCherry - CofilinS3E protein fusion

   Depositor

   Robert Hughes

   Article

   Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

   Insert

   Cry2PHR - mCherry - CofilinS3E
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK-FLAG-TRAF4

   Plasmid

   #58316

   Purpose

   FLAG-tagged mouse TRAF4

   Depositor

   Ying Zhang

   Article

   Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

   Insert

   TNF receptor associated factor 4 (Traf4 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-neo.ARRDC1

   Plasmid

   #38321

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   ARRDC1 (ARRDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR223-BFRF3

   Plasmid

   #195778

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BFRF3

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BFRF3
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTSlb-wt

   Plasmid

   #60728

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. binder/tag-WT-FAK-insertionB

   Plasmid

   #190440

   Purpose

   The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   FAK (Ptk2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SsrA inserted after aa 405

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR223-BKRF3

    Plasmid

    #195790

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BKRF3

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BKRF3
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-neo.ARRDC1-mCherry

    Plasmid

    #38319

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    ARRDC1 (ARRDC1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR223-BGLF2

    Plasmid

    #195800

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BGLF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BGLF2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoV-2-NSP3-c002

    Plasmid

    #173084

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tagSrc(WT)-mCer3 S208F/V224L

    Plasmid

    #179138

    Purpose

    mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Use

    Tags

    mCerulean3 (S208F/V224L)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-4T-1 3xHA-MKK6-EE

    Plasmid

    #47579

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK6-EE (MAP2K6 Human)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. PAmCherry1-N1-Lyn11

    Plasmid

    #202683

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    LYN(1-11):PAmCherry1 (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3611 pcDNA3 Bim

    Plasmid

    #8804

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2004 Oct 26;101

    Insert

    Bim (Bcl2l11 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-TRAF4

    Plasmid

    #58318

    Purpose

    EGFP-tagged mouse TRAF4

    Depositor

    Ying Zhang

    Article

    Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

    Insert

    TNF receptor associated factor 4 (Traf4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. HSC1-D4Z4-GiP

    Plasmid

    #58542

    Purpose

    Derived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lyn11C3S-EGFP

    Plasmid

    #202747

    Purpose

    weakly plasma membrane targeted fluorescent protein

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    LYN (1-11) (C3S):EGFP (LYN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNAS1b split mCherry 14-3-3β

    Plasmid

    #111880

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3β fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Actin.CIB.GFP

    Plasmid

    #133618

    Purpose

    Expresses beta actin - CIBN - GFP protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    betaActin - CIB
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNAS1b split mCherry 14-3-3σ

    Plasmid

    #111881

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Use

    Tags

    Split mCherry system: 14-3-3σ fused to C-terminal…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ActinS14V.CIB.GFP

    Plasmid

    #133620

    Purpose

    Expresses beta actin S14V - CIBN - GFP protein fusion

    Depositor

    Robert Hughes

    Article

    Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

    Insert

    betaActin - CIB
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pY71-tet.I.8

    Plasmid

    #133521

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV4-Flag-mIL-17RA/YFP.Hygro

    Plasmid

    #46862

    Purpose

    encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-YFP
    

    Use

    Tags

    Flag/IL-3 signal sequence and fused to CFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

28. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pGEMHE:hTRAAK(Q258K)

    Plasmid

    #133077

    Purpose

    X. laevis expression vector. It will generate the human TRAAK channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Q258K

    Promoter

    T7

    Available since

    Oct. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge