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Showing: 6181 - 6210 of 8073 results

  6. pSEVA63_Helper

    Plasmid
    #190432
    Purpose
    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Insert
    M13 genes: I-XI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    190432-plasmid-map-sequence-id-371816

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  11. pHEA

    Plasmid
    #168297
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.
    Insert
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    Use
    Tags
    6xHis, E-tag, and PelB signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168297-plasmid-map-sequence-id-334448

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  13. pAPV-DsRed2-ER-5

    Plasmid
    #170570
    Purpose
    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170570-plasmid-map-sequence-id-335779

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  17. tTA_BFP

    Plasmid
    #58854
    Purpose
    Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    tTA transcription factor fused to enhanced blue fluorescent protein
    Use
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58854-plasmid-map-sequence-id-327655

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  20. pGL4.23-MYC

    Plasmid
    #86461
    Purpose
    A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.
    Depositor
    Eric Lander
    Article
    Fulco et al Science. 2016 Sep 29. pii: aag2445.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MYC
    Available since
    Sept. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86461-plasmid-map-sequence-id-193100

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  24. Lyn-R-GECO1

    Plasmid
    #120410
    Purpose
    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.
    Depositor
    Won Do Heo
    Article
    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11
    Insert
    Lyn-R-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    120410-plasmid-map-sequence-id-247900

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  25. LeGO-iG2-wPRE-pA

    Plasmid
    #60489
    Purpose
    eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    IRES-eGFP; wPRE; BGH-pA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60489-plasmid-map-sequence-id-96860

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  27. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    71545-plasmid-map-sequence-id-132622

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  30. pZ8-P_dCas9

    Plasmid
    #74063
    Purpose
    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Propionate inducible promoter (prp)
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74063-plasmid-map-sequence-id-141970

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Showing: 6181 - 6210 of 8073 results