We narrowed to 14,534 results for: egf

1. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227901

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227902

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227905

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMLV-EGFP-P2A-Puro

   Plasmid

   #160226

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP-P2A-PuroR
   

   Use

   Retroviral

   Expression

   Bacterial and Mammalian

   Promoter

   LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-EGFP-Tau AP P301L

   Plasmid

   #46906

   Purpose

   expresses EGFP tagged Tau AP P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP P301L (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L AND all 14 S/P or T/P amino acid residues (…

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. cytoplasmic EKAR (EGFP-mRFP)

   Plasmid

   #18680

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

   Plasmid

   #169327

   Purpose

   Expression of CEP41-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag7 (CEP41 Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid

    #114404

    Purpose

    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

    Depositor

    Allen Institute for Cell Science

    Insert

    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTol-Promoter:NLS-tdMCP-eGFP

    Plasmid

    #86248

    Purpose

    For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Insert

    beta Actin promoter:NLS-tdMCPeGFP
    

    Use

    Used with transposase to make transient or stable…

    Tags

    NLS-tdMCP-eGFP

    Promoter

    Beta Actin

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFPC1-FLAG-Synaptojanin 1-170

    Plasmid

    #22294

    Depositor

    Pietro De Camilli

    Article

    Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCSC-GFP-NES-EGFP

    Plasmid

    #221810

    Purpose

    Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    GFP-NES-EGFP
    

    Use

    Lentiviral

    Tags

    NES (internal tag)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-puro-eGFP-HEC1

    Plasmid

    #114022

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

    Plasmid

    #233366

    Purpose

    hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Insert

    NES-Kinprola_PKA-mEGFP
    

    Use

    AAV

    Tags

    NES and mEGFP

    Promoter

    hSyn1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N3-FLuc-K21-Rluc_AH

    Plasmid

    #148847

    Purpose

    Mammalian Expression of K21-Rluc

    Depositor

    Elisa Izaurralde

    Insert

    K21-Rluc
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP

    Plasmid

    #187073

    Purpose

    Mitochondria surface expression of dHaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

    Insert

    TOM20-dHaloTag7
    

    Expression

    Mammalian

    Mutation

    dHaloTag7 = HaloTag7-D106A

    Available Since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGW1-mCherry-EGFP-PIM

    Plasmid

    #111759

    Purpose

    High expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition

    Depositor

    Lukas Kapitein

    Article

    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:

    Insert

    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    

    Expression

    Mammalian

    Mutation

    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

    Promoter

    CMV

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-U6-UL29-egfp-U3-UL8

    Plasmid

    #166694

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
    

    Use

    Lentiviral

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-neo FlucSM EGFP

    Plasmid

    #90171

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Expression

    Mammalian

    Mutation

    R188Q in FLuc

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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