We narrowed to 6,627 results for: hwa

1. pEHISSSO6206

   Plasmid

   #97065

   Purpose

   Expressing Sulfolobus solfataricus orf SSO6206 protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO6206
   

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmirGlo-CALB2-3'UTR delta ARE&mir30

   Plasmid

   #74430

   Purpose

   Calb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-1D4-mCherry

   Plasmid

   #73423

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1D4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1D4 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM6-4A6-mCherry

   Plasmid

   #73424

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4A6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P4A6 (orthogonal T7-lac variant)

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-1E4-mCherry

   Plasmid

   #73426

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1E4 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1E4 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-1B6-mCherry

   Plasmid

   #73420

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 1B6 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P1B6 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-3H5-mCherry

   Plasmid

   #73419

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3H5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3H5 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. -161+80pCalb2/mutE2F(-69)-like

   Plasmid

   #66743

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, mutant)

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   GCG (-69,-68,-67) to ATT

   Promoter

   Calb2

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RalA (G23V)(mature peptide)-pcw107-V5

   Plasmid

   #64643

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   G23V

   Promoter

   PGK

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. RalA (G23V)(mature peptide)-pcw107

    Plasmid

    #64642

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    RALA (RALA Human)
    

    Use

    Lentiviral

    Mutation

    G23V

    Promoter

    PGK

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

11. tdEos-EB3-7

    Plasmid

    #57610

    Purpose

    Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    Kris Wood, David Sabatini

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    V617F

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNK3071

    Plasmid

    #219755

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Expression

    Plant

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-IP-mCherry-TOSI

    Plasmid

    #172496

    Purpose

    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    PDCD4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    Mcherry

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK497

    Plasmid

    #219754

    Purpose

    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    

    Expression

    Plant

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-IP-mVenus-TOSI

    Plasmid

    #172491

    Purpose

    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

    Depositor

    David Scadden

    Article

    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

    Insert

    Pdcd4 (Pdcd4 Mouse)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    MLV LTR

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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