We narrowed to 5,829 results for: pcas

1. p206_LTJ_sgRNACD45.2_R3

   Plasmid

   #82674

   Purpose

   sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD45.2 region 3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX-H2B-KikGR

   Plasmid

   #98649

   Purpose

   Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

   Insert

   KikGR
   

   Tags

   H2B

   Expression

   Mammalian

   Promoter

   CMV-IE enhancer chick beta actin promoter

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pTRANS_220d

   Plasmid

   #91114

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRANS_232

   Plasmid

   #91121

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Promoter

   AtRPS5A

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

   Plasmid

   #183027

   Purpose

   B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ABE-RA2.0

   Plasmid

   #208299

   Purpose

   Expresses ABE-RA2.0 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA2.0-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABE-RA3.1

   Plasmid

   #208302

   Purpose

   Expresses ABE-RA3.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA3.1-SpCas9 D10A
   

   Expression

   Mammalian

   Mutation

   E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFPL-Ch

    Plasmid

    #61647

    Purpose

    Gateway vector for generating cyan fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    enhanced cyan fluorescent protein
    

    Expression

    Plant

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGJJ001

    Plasmid

    #183619

    Purpose

    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFPL-Rh

    Plasmid

    #61649

    Purpose

    Gateway vector for generating red fluorescent protein fusions in fungi

    Depositor

    Mark Farman

    Article

    Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

    Insert

    monomeric red fluorescent protein
    

    Expression

    Plant

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-S12

    Plasmid

    #84031

    Purpose

    To episomally express codon optimized Cas9 and chimeric guide RNA

    Depositor

    Sen Wu

    Article

    Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

    Inserts

    hSpCas9
    eGFP
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    CMV and CMV (downstream of F2A self-cleaving pept…

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRANS_210d

    Plasmid

    #91109

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSQT817

    Plasmid

    #53373

    Purpose

    wildtype Cas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRANS_201

    Plasmid

    #91105

    Purpose

    Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    No Promoter

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMP116

    Plasmid

    #207918

    Purpose

    GreenBraid destination vector pGBZ1 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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