We narrowed to 31,661 results for: uros

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pOC4 Grin1-smFP-V5 KI

Plasmid

#183439

Purpose

FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP V5 donor

Use

CRISPR

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Gria1-GFP KI

Plasmid

#183430

Purpose

CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP donor

Use

CRISPR

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY11

Plasmid

#191017

Purpose

Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

Ultrapanneuronal

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SL016: AAV-CAG-FLEX-CheRiff-GFP

Plasmid

#124776

Purpose

For AAV based expression of CheRiff in Cre-Positive cells

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

CheRiff-GFP

Use

AAV and Cre/Lox

Tags

GFP

Promoter

CAG

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only
RabV CVS-N2c(deltaG)-APEX2-Venus

Plasmid

#73467

Purpose

Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

APEX2-Venus

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p194-RV-CAG-dio-mScarlet-T2A-Brn2

Plasmid

#204741

Purpose

Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Brn2

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-I-Sce1-slc6a3[11kb]:egfp

Plasmid

#53240

Purpose

allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT-GFP

Use

Cloning

Tags

GFP

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p254-RV-CAG-dio-mScarlet-T2A-Dach1-202

Plasmid

#204742

Purpose

Conditional expression of target gene Dach1 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Dach1

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p186-RV-CAG-dio-mScarlet-T2A-mVenus

Plasmid

#204740

Purpose

Conditional expression of target gene mVenus with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mVenus

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-ND1-K139D-T2A-mCherry

Plasmid

#178587

Purpose

Expression of NEUROD1-K139D and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1-K139D (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Mutation

NEUROD1-K139D

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-ND1-R111L-T2A-mCherry

Plasmid

#178586

Purpose

Expression of NEUROD1-R111L and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1-R111L (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Mutation

NEUROD1-R111L

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-GFP-shRNA-scramble

Plasmid

#182503

Purpose

Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

hSyn promoter

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-iCre-2A-venus

Plasmid

#182499

Purpose

Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

iCre-2A-Venus

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

fragment of histidine decarboxylase gene promoter…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

encodes only the first 68 amino acids of Tac1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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