We narrowed to 5,415 results for: PEP;
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Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC70
Plasmid#118878Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, cleavable with TEV protease; HIS6, cleavabl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-HA-Ago2
Plasmid#115362PurposeExpression vector to produce HA-tagged human Ago2 protein without N-peptideDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 NFKB1
Plasmid#67126PurposeStandard for cell-free expression benchmarking.DepositorInsertNFKB1 (NFKB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
UseTags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
rat APOBEC1-EGFP
Plasmid#112857Purposeplasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion proteinDepositorInsertapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST47-CypB-Del3 C-term GFP
Plasmid#36136DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 39-216 of cyclophilin B; G63…PromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-human MYCN-V421D
Plasmid#74166PurposeMammalian expression of human MycN-VD mutantDepositorInsertMycN (MYCN Human)
UseTagsHAExpressionMammalianMutationV421DPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC92
Plasmid#118910Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagseGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC6
Plasmid#118864Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFPDepositorInsertNcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, EGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease; GST tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC204
Plasmid#118894Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and mKate2, HIS6,…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-EGFP-FF2
Plasmid#164922PurposeExpresses EGFP cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTG03-AmpFRT
Plasmid#128272PurposeTIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG05-Amp-FRT
Plasmid#128274PurposeTIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
PcDNA-DEST53-CypB-Nucl N-term GFP
Plasmid#36123DepositorInsertcyclophilin B (PPIB Human, contains CypB AA 33-216)
UseTagsGFPExpressionMammalianMutationA212SPromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOCC215
Plasmid#118879Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
UseTagsDendra2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only