We narrowed to 22,077 results for: his

1. pGEX-THTAP-CALPAIN-7 1-165

   Plasmid

   #180610

   Purpose

   Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-65

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJPT011-ZF6 1x FLAG-synTF-PPF

   Plasmid

   #202501

   Purpose

   Express "PPF" synTF (ZF6)

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   p65-ZF6
   

   Use

   Tags

   1x FLAG and Palmitoylation-Palmitoylation-Farnesy…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJPT025-24 Angstrom 12TMD de novo_mRFP1_3x FLAG

   Plasmid

   #202507

   Purpose

   Express 24 Angstrom 12TMD de novo protein

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   24 Angstrom 12TMD de novo
   

   Use

   Tags

   mRFP1-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJPT026-32 Angstrom 4TMD de novo_mRFP1_3x FLAG

   Plasmid

   #202508

   Purpose

   Express 32 Angstrom 4TMD de novo protein

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   32 Angstrom 4TMD de novo
   

   Use

   Tags

   mRFP1-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJPT028-40 Angstrom 12TMD de novo_mRFP1_3x FLAG

   Plasmid

   #202510

   Purpose

   Express 40 Angstrom 12TMD de novo protein

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   40 Angstrom 12TMD de novo
   

   Use

   Tags

   mRFP1-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJPT024-24 Angstrom 4TMD de novo_mRFP1_3x FLAG

   Plasmid

   #202506

   Purpose

   Express 24 Angstrom 4TMD de novo protein

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   24 Angstrom 4TMD de novo
   

   Use

   Tags

   mRFP1-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJPT027-40 Angstrom 4TMD de novo_mRFP1_3x FLAG

   Plasmid

   #202509

   Purpose

   Express 40 Angstrom 4TMD de novo protein

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   40 Angstrom 4TMD de novo
   

   Use

   Tags

   mRFP1-3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJPT009-ZF6 PM-synTF-1x FLAG

   Plasmid

   #202499

   Purpose

   Express "PM" synTF (ZF6)

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   p65-ZF6
   

   Use

   Tags

   1x FLAG and Palmitoylation-Myristoylation Site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJL1-AcrA

   Plasmid

   #198214

   Purpose

   pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   AcrA
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRD461

    Plasmid

    #178198

    Purpose

    Rok with a C-terminal His-tag overexpression

    Depositor

    Remus Dame

    Article

    Erkelens et al Nucleic Acids Res. 2022 Nov 21. pii: 683

    Insert

    rok 6xHis
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FnRPR9-10

    Plasmid

    #182568

    Purpose

    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 9-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FnRPR8-10

    Plasmid

    #182570

    Purpose

    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

    Depositor

    Timothy Springer

    Article

    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

    Insert

    Human fibronectin fragment, type-III repeats 8-10
    

    Use

    Tags

    His and TEV cleavage site after His tag

    Expression

    Bacterial

    Mutation

    R1374A, P1376A, R1379A

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL2196

    Plasmid

    #170400

    Purpose

    For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-717, replaced with 114-130)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2197

    Plasmid

    #170401

    Purpose

    For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-716, replace with 347-360)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2198

    Plasmid

    #170402

    Purpose

    For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-716, replace with 580-600)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2251

    Plasmid

    #170403

    Purpose

    For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 225-249)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2252

    Plasmid

    #170404

    Purpose

    For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-716, replace with 455-479)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2253

    Plasmid

    #170405

    Purpose

    For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    8xHis2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 812-836)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ppih.001.177.W133A

    Plasmid

    #137655

    Purpose

    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Melissa Jurica, Tara Davis

    Article

    Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

    Insert

    ppih (PPIH Human)
    

    Use

    Tags

    GST-(His)6-thrombin

    Expression

    Bacterial

    Mutation

    001-177-W133A

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid

    #179528

    Purpose

    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 R1333A/R1335A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed arginines 1333 and 1335 to alanine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM33_SPSB1_SPRY

    Plasmid

    #178487

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SPSB1_SPRY (SPSB1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETM33_YES1_SH3

    Plasmid

    #178492

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    YES1_SH3 (YES1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AlkB-D135G

    Plasmid

    #160434

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Glycine; deleted the…

    Promoter

    T7

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDB367

    Plasmid

    #110277

    Purpose

    gHEEE_02 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHEEE_02
    

    Use

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSKB3-SaGGR-F219L (JBEI-7186)

    Plasmid

    #110151

    Purpose

    pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

    Depositor

    Jay Keasling

    Article

    Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

    Insert

    GGR
    

    Use

    Tags

    TEV-cleavable His-tag

    Expression

    Bacterial

    Mutation

    SaGGR-F219L mutant gene

    Promoter

    T7

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p5A1-T

    Plasmid

    #92017

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p415 δCLD2α

    Plasmid

    #73874

    Purpose

    δCLD2α - His tagged (μHD deleted)

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Arcn1/ δCLD2α (ARCN1 Bovine)
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p5C3-T

    Plasmid

    #92022

    Purpose

    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Expression

    Mutation

    S219V

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBacPAK-HFT-dKDM4A-H195A

    Plasmid

    #20236

    Depositor

    Jerry Workman

    Article

    Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

    Insert

    dKDM4A (Kdm4A Fly)
    

    Use

    Tags

    FLAG, His, and TEV

    Expression

    Insect

    Mutation

    changed His 195 to Ala

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only