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pCY 3010-05

Plasmid

#36211

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

LEU2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-LEU2

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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TOPO-BC43

Plasmid

#84437

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC43

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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HuEV-A

Plasmid

#68342

Purpose

Gateway compatible human expression vector that adds a "flexible" 3XFLAG,V5,YFP N terminus tag

Depositor

Jef Boeke

Article

Mita et al Biol Proced Online. 2016 Aug 15;18:16. d

Type

Empty backbone

Use

Cre/Lox

Tags

3xFLAG, V5 and YFP

Expression

Mammalian

Promoter

TET-CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTHSSd_23

Plasmid

#59954

Purpose

PTac T7 sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBY2946

Plasmid

#23223

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi

Expression

Worm

Available Since

May 4, 2010

Availability

Academic Institutions and Nonprofits only

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pCY 3090-02

Plasmid

#36231

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yEpolylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-mCherry-hphMX4

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTHSSd_44

Plasmid

#59966

Purpose

constitutive expression of T7 sigma fragment

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

Pcon sigma

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pKB234.2

Plasmid

#170023

Purpose

Encodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
hBD3 Δ alpha-helix

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Dec. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pTHSSd_25

Plasmid

#59956

Purpose

PTac K1FR sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

K1FR sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_26

Plasmid

#59957

Purpose

PTac CGG sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

CGG sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mV-CrPH-LOV1_227

Plasmid

#58687

Purpose

Expresses mVenus-fusion protein of CrPH-LOV1 domain (Chlamydomonas reinhardtii Phototropin)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-CrPH-LOV1

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2442

Plasmid

#36833

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SS18-TALEN_Left (Ss18 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pTHSSd_29

Plasmid

#59959

Purpose

PTac alpha fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

alpha fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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TAL2350

Plasmid

#36769

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

XPC-TALEN_Left (Xpc Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
mV-RsLP-LOV_367

Plasmid

#58690

Purpose

Expresses mVenus-fusion protein of RsLP-LOV domain (Rhodobacter sphaeroides ATCC 17025 protein)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-RsLP-LOV

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_1

Plasmid

#59947

Purpose

Splitposon in KanR ColE1 backbone

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Splitposon w/ KanR, LacI & PTac

Expression

Bacterial

Mutation

N/A

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mV-NcWC1-LOV_368

Plasmid

#58691

Purpose

Expresses mVenus-fusion protein of NcWC1-LOV domain (Neurospora crassa White Collar-1)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-NcWC1-LOV

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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mV-AtPH1-LOV2_229

Plasmid

#58688

Purpose

Expresses mVenus-fusion protein of AtPH1-LOV2 domain (Arabidopsis thaliana Phototropin 1)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-AtPH1-LOV2

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVd2-mCherry-Dr-TrkA

Plasmid

#127957

Purpose

Myr mCherryDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkA

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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TAL2374

Plasmid

#36785

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SETDB1-TALEN_Left (Setdb1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pTHSSd_24

Plasmid

#59955

Purpose

PTac T3 sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T3 sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pN23-LIC-His-FLAG

Plasmid

#165478

Purpose

pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2330

Plasmid

#36756

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MSH2-TALEN_Left (Msh2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pXcX-G4BS-3.6TPH-EGFP

Plasmid

#32572

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBY2807

Plasmid

#23222

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

Gateway-SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only