We narrowed to 9,202 results for: sacs
-
Plasmid#115431PurposeURA3-marked RPS28DepositorInsertsUseTagsExpressionYeastMutationKpnI site in intronPromoterAshbya gossypii and endogenousAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pEPOR2KN0080
Plasmid#117647PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0076
Plasmid#117643PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0062
Plasmid#117629PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)DepositorInsert[35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0061
Plasmid#117628PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_UUAT1 {AT5G04160}(pEPOR1CB0087)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0012
Plasmid#117533PurposeLevel0 Golden Gate part: CDS, eSaCas9 (no stop codon)DepositorInserteSaCas9 (no stop codon)
UseCRISPRTagsExpressionMutationR499A,Q500K,R654A,G655RPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-YJR129c
Plasmid#85118Purposebacterial expression of YJR129cDepositorInsertEFM3 (EFM3 Budding Yeast)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Spt2-K166R-GFP
Plasmid#115573PurposeExpresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166DepositorInsertSPT2
UseTagsGFPExpressionBacterial and YeastMutationLysine 166 mutated to ArgininePromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316Nab3-11
Plasmid#110535PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationtwo missense mutations creating Nab3-11PromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3-11
Plasmid#110534PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationtwo missense mutations creating Nab3-11PromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH846-gst-dissc
Plasmid#96907PurposePlasmid for yeast expression of GST encoded by non-preferred codonsDepositorArticleInsertyeast codon dis-optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
UseTags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mECitrine
Plasmid#105153PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mECitrine
UseTags81nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter81nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
P415 δCLD+Ret2 2α
Plasmid#73878PurposeSwapped helicesDepositorInsertδCLD+Ret2 2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
P415 Ret2LD+δC2α
Plasmid#73877PurposeSwapped helicesDepositorInsertRet2LD+δC2α
UseTagsHisExpressionYeastMutationPromoterMet25Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415TEF-NQYFP
Plasmid#99554PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335R
Plasmid#87299PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335RDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335RPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-CLF1
Plasmid#87548PurposeEncodes Y2H Gal4 DNA binding domain for Clf1DepositorInsertCLF1 (CLF1 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 D450G
Plasmid#87303PurposeEncodes Y2H Gal4 activation domain for Hsh155 D450GDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationD450GPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 P369K
Plasmid#87302PurposeEncodes Y2H Gal4 activation domain for Hsh155 P369KDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationP369KPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only