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Plxna1-AP-His

Plasmid

#71996

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac_His-ZZ-TEV-Lis1-SNAPf

Plasmid

#133073

Purpose

Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

Depositor

Simon Bullock, Andrew Carter

Article

Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

Insert

Lis1 (PAFAH1B1 Human)

Tags

8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-FL

Plasmid

#128415

Purpose

Express INTS3 full-length in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

full-length

Promoter

tac promoter

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/MPL-V5

Plasmid

#204531

Purpose

Mammalian expression of human MPL-V5

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-V5 (MPL Human)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ASB2

Plasmid

#207185

Purpose

doxycycline-inducible expression of Human ASB2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ASB2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pBa-FRB-3myc-Rab7

Plasmid

#64210

Purpose

Rab7 with N-terminal FRB and triple myc tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Rab7 (Rab7 Mouse)

Tags

FRB and triple myc tag (3myc)

Expression

Mammalian

Promoter

chicken Beta Actin

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL

Plasmid

#187712

Purpose

Express GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pQCX-CD28 WT

Plasmid

#223640

Purpose

Retroviral expression of mouse CD28 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (Cd28 Mouse)

Use

Retroviral; mammalian expression

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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Plxna3-Fc-His

Plasmid

#72124

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-Mito-7

Plasmid

#57189

Purpose

Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

Insert

Mito (COX8A Human)

Tags

PA-mCherry1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUDC175

Plasmid

#103019

Purpose

S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pY003 (pFnCpf1_delta Cas)

Plasmid

#69974

Purpose

Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus without Cas genes

Use

CRISPR

Expression

Bacterial

Mutation

Cas genes are deleted from locus

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2CS

Plasmid

#12284

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2CS (PTPN11 Human)

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 459 mutated to Serine

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pENTR4 CDS A. thaliana PHOT1

Plasmid

#209187

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT3G45780.1 (PHOT1 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-Ago2

Plasmid

#74231

Purpose

GFP-Ago2 lentiviral vector

Depositor

Carl Novina

Article

Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

Insert

Ago2 (AGO2 Human)

Use

Lentiviral

Tags

eGFP

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
pLINK cyclin T1 (1-280) myc (P#586)

Plasmid

#14612

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (CCNT1 Human)

Tags

myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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