We narrowed to 14,039 results for: TIM

1. ptet-lux-Tn7T-Gm

   Plasmid

   #226434

   Purpose

   This vector features a tetracycline-inducible system to regulate luxABCDE reporter expression, optimized for efficiency in P. aeruginosa.

   Depositor

   Xue Liu

   Article

   Zhang et al Nat Commun. 2025 Jul 1;16(1):5870. doi:

   Inserts

   optimized TetR
   luxABCDE
   

   Expression

   Bacterial

   Promoter

   Pcon and Ptet

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIN3 (gpdA-cTerm-Citrine2-ptrA)

   Plasmid

   #188737

   Purpose

   Expresses Citrine2 for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-Citrine2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   Citrine2

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIN4 (gpdA-nTerm-Citrine2-ptrA)

   Plasmid

   #188738

   Purpose

   Expresses Citrine2 for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-Citrine2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   Citrine2

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIN5 (gpdA-cTerm-mTurqoise2-ptrA)

   Plasmid

   #188739

   Purpose

   Expresses mTurqoise2 for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mTurquoise PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mTurquoise

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIN6 (gpdA-nTerm-mTurqoise2-ptrA)

   Plasmid

   #188740

   Purpose

   Expresses mTurqoise2 for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mTurqoise2 PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUt-MONARCH 1.0_crEGFP

   Plasmid

   #224790

   Purpose

   LPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selection

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crEGFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV-d2i

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUt-MONARCH 2.0_crEGFP

   Plasmid

   #224793

   Purpose

   LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   crEGFP
   Triplex stablier
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV-d2i

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-hU6-2O-crBFP_EF1a-BFP

   Plasmid

   #224783

   Purpose

   BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-hU6-2O-crEGFP_EF1a-BFP

   Plasmid

   #224784

   Purpose

   EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crEGFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6 and hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28:LanIV-Streptomyces

    Plasmid

    #226250

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIV-Streptomyces)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid

    #169134

    Purpose

    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Fgß-FIVAR-ELP-HIS-ybbR

    Plasmid

    #169136

    Purpose

    E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    FIVAR (found in various architectures)
    

    Tags

    3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid

    #169133

    Purpose

    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    

    Tags

    3xELP linker, 6x Histag, and ybbr tag

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSuperior.retro.neo GFP shDNM1L

    Plasmid

    #220356

    Purpose

    Retroviral expression vector for an shDNM1L

    Depositor

    David Kashatus

    Article

    Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

    Insert

    shDNM1L (DNM1L Human)
    

    Use

    RNAi and Retroviral

    Promoter

    H1

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28:LanII-56

    Plasmid

    #225073

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-56)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28:LanII-2C

    Plasmid

    #225070

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2C)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28:LanII-2A

    Plasmid

    #225068

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII-2A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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