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Showing: 4161 - 4180 of 6925 results
  1. pCDH-DCX-TurboID-V5-puro

    Plasmid
    #190742
    Purpose
    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
    Depositor
    Insert
    DCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. STARR-seq luciferase validation vector_ORI_SV40

    Plasmid
    #99309
    Purpose
    Luciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    SV40 enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pCDH-EMTB-TurboID-V5-puro

    Plasmid
    #190741
    Purpose
    A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Insert
    EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Insert
    Gαs-LgB113 (GNAS Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid
    #140019
    Purpose
    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration
    Depositor
    Insert
    EcoLeuRS (AnapRS)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…
    Promoter
    EF1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 4161 - 4180 of 6925 results