We narrowed to 443 results for: DHFR
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Plasmid#179731PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sitesDepositorInserthuman DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
UseRetroviralTagsHiBiTPromoterLTR/MMLVAvailable SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
ExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KK601: pMVP (L3-L2) ecDHFR DD + polyA
Plasmid#121790PurposepMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.DepositorInsertecDHFR Degron Domain + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-NLC-DHFR-SpCas9
Plasmid#124523PurposeExpresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cellsDepositorInsertNLC-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)
Plasmid#178850PurposeMammalian expression of EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)
ExpressionMammalianPromoterCAGAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Kif1A(1-489)-DHFR-myc
Plasmid#117833PurposeConstitutively active Kinesin-3 motor tagged with DHFR and mycDepositorInsertKif1A (1-489) (Kif1a Mouse)
TagsDHFR and mycMutationonly aa 1-489 (motor domain)PromoterCMVAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cDHFR174
Plasmid#188256PurposePlasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
nDHFR173-gs10-NLS-VPR
Plasmid#188262PurposePlasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnDHFR173 (DHFR Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
RGG-mCherry-RGG-eDHFR
Plasmid#164648PurposeTwo RGG domains tagged with mCherry and eDHFRDepositorInsertRGG
UseCre/LoxExpressionMammalianPromoterCAGAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-Cit-tvmvs-DHFR
Plasmid#116060PurposeReporter plasmid for measuring protease activityDepositorInsertCit-tvmvs-DHFR
ExpressionMammalianAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only