We narrowed to 4,504 results for: pcr

1. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRII-Topo Cav1 in situ probe

   Plasmid

   #45624

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Cav1 in situ probe (Cav1 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 1472-2124 of AB029929.1

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCru5-/GCCACC-mEGFP-IRES-mCherry

   Plasmid

   #49226

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRII-Blunt-U6-HES7-STOP-gRNA

   Plasmid

   #130933

   Purpose

   gRNA for tag human HES7

   Depositor

   James Thomson

   Article

   Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

   Insert

   HES7-STOP-gRNA (HES7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCru5-/CGA-mEGFP-IRES-mCherry

   Plasmid

   #49224

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRII-HSV2.3 TCR beta chain

   Plasmid

   #74077

   Purpose

   gBT-I TCR beta chain cDNA in pCRII

   Depositor

   Francis R. Carbone

   Article

   Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

   Insert

   TCR beta cDNA clone HSV2.3 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR3-vsv-GCN4-dCEN-INCENP

   Plasmid

   #108510

   Purpose

   expression of vsv-GCN4-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   GCN4-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   replace CEN box for GCN4 dimerazation domain

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRII-Topo Ptn in situ probe

   Plasmid

   #45632

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Ptn in situ probe (Ptn Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 1241-1830 of NM_008973

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pCru5-/UAA-mEGFP-IRES-mCherry

   Plasmid

   #49225

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-Topo S100a10 in situ probe

    Plasmid

    #45603

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    s100a10 in situ probe (S100a10 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 21-275 of BC025044

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCRII-Topo Foxp2 in situ probe

    Plasmid

    #45620

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Foxp2 in situ probe (Foxp2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp#1709-2142 of AF339106

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRII-Topo Cux2 in situ probe

    Plasmid

    #45623

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Cux2 in situ probe (Cux2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 1069-1694 on NM_007804

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCRII-Topo Gp88 in situ probe

    Plasmid

    #45628

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Gpr88 in situ probe (Gpr88 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 2742-3255 of AB042408.1 or …

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCRII-Topo Epha3 in situ probe

    Plasmid

    #45627

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Epha3 in situ probe (Epha3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 3179-3773 of NM_010140

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-Topo Nectin-3 in situ probe

    Plasmid

    #45633

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Nectin-3 in situ probe (Nectin3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1626-2039 of NM_021495

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCRII-Topo Crym in situ probe

    Plasmid

    #45601

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Crym in situ probe (Crym Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 756-1191 of NM_016669

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCR3-vsv-AuroraB L154G/H250Y

    Plasmid

    #108490

    Purpose

    expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRII-Topo Cdh10 in situ probe

    Plasmid

    #45638

    Depositor

    Jeffrey Macklis

    Insert

    Cdh10 in situ probe (Cdh10 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 2628-3061 of Cdh10 (GenBank …

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCRII-Topo Nnmt in situ probe

    Plasmid

    #45631

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Nnmt in situ probe (Nnmt Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 454-908 of NM_010924.2

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pCRII-Topo Diap3 in situ probe

    Plasmid

    #45602

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Diap3 in situ probe (Diaph3 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 1589-2321 of BC028920

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pCRII-Topo Crim1 in situ probe

    Plasmid

    #45600

    Depositor

    Jeffrey Macklis

    Article

    Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

    Insert

    Crim1 in situ probe (Crim1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    insert contains bp#4062-4502 of NM_015800.3

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pCRII-Topo Chn2 in situ probe

    Plasmid

    #45625

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    Chn2 in situ probe (Chn2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 2212-2619 of BC051139.1

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

    Plasmid

    #235608

    Purpose

    Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRII-TOPO-dgat1a in situ probe

    Plasmid

    #232137

    Purpose

    zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1a (dgat1a Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRII-TOPO-dgat1b in situ probe

    Plasmid

    #232138

    Purpose

    zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1b
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRII-TOPO-mogat2 in situ probe

    Plasmid

    #232139

    Purpose

    zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat2 (mogat2 Zebrafish)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCR4-beta-globin::lacZ-H11

    Plasmid

    #227000

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

    Depositor

    Guillaume Andrey

    Article

    Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-globin

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR 2.1-TOPO Fzd6 N352A HA

    Plasmid

    #208365

    Purpose

    Extracellular HA-tagged Fzd6 N352A (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Asparagine 352 to Alanine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR 2.1-TOPO Fzd6 S354I HA

    Plasmid

    #208364

    Purpose

    Extracellular HA-tagged Fzd6 S354I (mouse)

    Depositor

    Maureen Cetera, Danelle Devenport

    Article

    Cetera et al Development. 2023 Aug 25:dev.202078. doi

    Insert

    Fzd6 (Fzd6 Mouse)
    

    Use

    Cloning vector

    Tags

    HA

    Expression

    Mutation

    Serine 354 to Isoleucine

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge