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Showing: 801 - 840 of 3893 results

  6. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid
    #140271
    Purpose
    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    140271-plasmid-map-sequence-id-317910

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  7. pGLTR-S-PURO

    Plasmid
    #58244
    Purpose
    GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and no
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58244-plasmid-map-sequence-id-89655

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  10. Donor_eGP2AP_RC

    Plasmid
    #133784
    Purpose
    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.
    Depositor
    Sriram Kosuri
    Article
    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d
    Type
    Empty backbone
    Use
    Tags
    eGFP-P2A-Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133784-plasmid-map-sequence-id-274492

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  11. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  20. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid
    #157732
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nprot-HF_GC3opt (N SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157732-plasmid-map-sequence-id-308146

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  21. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  25. pLyGo-Ec-2

    Plasmid
    #163128
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163128-plasmid-map-sequence-id-331492

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  27. pLyGo-Ec-8

    Plasmid
    #163136
    Purpose
    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P(rhaB)
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163136-plasmid-map-sequence-id-332138

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  28. pLyGo-Ec-6

    Plasmid
    #163132
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163132-plasmid-map-sequence-id-338662

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  29. 1114H

    Plasmid
    #200640
    Purpose
    An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males
    Depositor
    Omar Akbari
    Article
    Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.
    Insert
    gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200640-plasmid-map-sequence-id-405636

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  30. pLyGo-Ec-4

    Plasmid
    #163130
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163130-plasmid-map-sequence-id-338661

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  31. pLyGo-Ec-1

    Plasmid
    #163127
    Purpose
    pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Ubiquitin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    163127-plasmid-map-sequence-id-331491

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  32. pLyGo-Ec-3

    Plasmid
    #163129
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163129-plasmid-map-sequence-id-332101

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  33. pLyGo-Ec-5

    Plasmid
    #163131
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163131-plasmid-map-sequence-id-329722

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  37. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    87067-plasmid-map-sequence-id-192710

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  38. pcDNA3.1-ccdB-Renilla

    Plasmid
    #87065
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87065-plasmid-map-sequence-id-173589

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  39. pcDNA3.1-ccdB-Firefly

    Plasmid
    #87066
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    87066-plasmid-map-sequence-id-190852

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  40. pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

    Plasmid
    #128455
    Purpose
    lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128455-plasmid-map-sequence-id-256467

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Showing: 801 - 840 of 3893 results