We narrowed to 3,024 results for: ER

1. AAV-hSyn-ASAP3ER

   Plasmid

   #195358

   Purpose

   ASAP3ER under control of human synapsin promoter

   Depositor

   Brenda Bloodgood

   Article

   Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

   Insert

   ASAP3ER
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. VPS13D(∆ATG2-C/PH)^EGFP

   Plasmid

   #194004

   Purpose

   Deletion of DHPH domain of VPS13D

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D (VPS13D Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   early stop codon before DHPH

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-Rtn2b-R60f-mCherry

   Plasmid

   #186607

   Purpose

   Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 mutant R60f (RTN2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   c.178insC (R60f)

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-Rtn2b-mNeon-TurboID

   Plasmid

   #186612

   Purpose

   Third generation lentiviral vector expressing full-length Reticulon 2 fused to mNeonGreen and V5-TurboID

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B (RTN2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Rtn2b-NTD1-271-mNeon

   Plasmid

   #186601

   Purpose

   Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 271 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 1 to 271 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Rtn2b-NTD1-135-mNeon

   Plasmid

   #186602

   Purpose

   Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 135 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 1 to 135 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rtn2b-NTD1-60-mNeon

   Plasmid

   #186603

   Purpose

   Encodes human Reticulon 2 isoform B N-terminal domain aa 1 to 60 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 1 to 60 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rtn2b-NTD136-271-mNeon

   Plasmid

   #186604

   Purpose

   Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMpGE_En03-sgRNA-Target1 (MpRTN1)

   Plasmid

   #186291

   Purpose

   Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   sgRNA-Target1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-doraWT_rescue_construct

    Plasmid

    #190608

    Purpose

    Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc-sgRNA-Cas9.dora_1

    Plasmid

    #190700

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc-sgRNA-Cas9.dora_2

    Plasmid

    #190701

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc-sgRNA-Cas9.dora_3

    Plasmid

    #190702

    Purpose

    Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    dora (CG34401) sgRNA 3
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

    Plasmid

    #158771

    Purpose

    Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers, ADVVCC/QMSY cleavage site

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGPN

    Plasmid

    #187482

    Purpose

    Expression vector - GFP

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLENTI CMV GFP-TREX1 P290L BLAST

    Plasmid

    #164239

    Purpose

    lentiviral plasmid for GFP-TREX1 P290L

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    P290L point mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLENTI CMV GFP-TREX1 Y305C BLAST

    Plasmid

    #164240

    Purpose

    lentiviral plasmid for GFP-TREX1 Y305C

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y305C point mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLENTI CMV GFP-TREX1 D272fs BLAST

    Plasmid

    #164237

    Purpose

    lentiviral plasmid for GFP-TREX1 D272fs

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D272 frame shift mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLENTI CMV GFP-TREX1 L287fs BLAST

    Plasmid

    #164238

    Purpose

    lentiviral plasmid for GFP-TREX1 L287fs

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L287 frame shift mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-HA-PGRMC1-1-54

    Plasmid

    #173472

    Purpose

    PGRMC1 lacking the C-terminal region downstream of the transmembrane domain

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 55-194

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-HA-PGRMC1-21-195

    Plasmid

    #173473

    Purpose

    PGRMC1 lacking the N-terminal region upstream of the transmembrane region

    Depositor

    Bill Tsai

    Article

    Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

    Insert

    PGRMC1 (PGRMC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted for amino acids 1-20

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pQCXIP-GFP-BAF

    Plasmid

    #164248

    Purpose

    retroviral plasmid for GFP-BAF

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    BAF (BANF1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLENTI CMV GFP-TREX1 G306A BLAST

    Plasmid

    #164241

    Purpose

    lentiviral plasmid for GFP-TREX1 G306A

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G306A point mutation

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. IBMc318

    Plasmid

    #161941

    Purpose

    Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-G-ER-LBD-S-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDOC-K-glmS-GFPrev

    Plasmid

    #158060

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBz106_CsoS2_MR

    Plasmid

    #140843

    Purpose

    His-tagged CsoS2 Middle Region (MR), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 Middle Region (MR)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    only amino acids 257-603

    Promoter

    T7

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLz75_CB_operon_CsoS2_N3-N4

    Plasmid

    #140857

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-109 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLz76_CB_operon_CsoS2_N4

    Plasmid

    #140858

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-189 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBz109_CsoS2_NTD

    Plasmid

    #140842

    Purpose

    His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 N-terminal domain (NTD)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 262-869

    Promoter

    T7

    Available since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Use

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBz110_CsoS2_CTD

    Plasmid

    #140844

    Purpose

    His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 C-terminal domain (CTD)
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-594

    Promoter

    T7

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pProEx-Htb-cytATL2-6Gly-sfGFP

    Plasmid

    #124065

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTorPE-Y-GECO2m

    Plasmid

    #105066

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pILGFPJ1

    Plasmid

    #83561

    Purpose

    Express yEGFP-ERG9 ER signal peptide under the control of TEF1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    TEF1 promoter-yEGFP-ERG9 ER signal peptide
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Fast-GCaMP-RS09

    Plasmid

    #52649

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS09
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L414T mutated from GCaMP3

    Promoter

    T7

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

36. Fast-GCaMP-RS05

    Plasmid

    #52646

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS05
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M373Q mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

37. Fast-GCaMP-RS08

    Plasmid

    #52648

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-RS08
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T412Q/L414T mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

38. roGFP_iR_pQE30

    Plasmid

    #48634

    Purpose

    Bacterial expression vector encoding for the 6XHis tagged redox probe roGFPiR (roGFP C147, R147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    roGFP1
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    insertion R147B

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFS320-USP44-catDead

    Plasmid

    #43901

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    C282A, T91A

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pFS322-USP44-catDeadMyc

    Plasmid

    #43904

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    C282A, T91A

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pFS321-pDestMyc-USP44catDead

    Plasmid

    #43902

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C282A

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

42. pTorPE-Y-GECO2s

    Plasmid

    #105067

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTorPE-Y-GECO2.1s

    Plasmid

    #105068

    Purpose

    A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2.1s
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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44. pFSW Syt1-C2B R398,399Q IRES GFP

    Plasmid

    #133819

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    R398,399Q

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFSW Syt1-C2B K326,327E IRES GFP

    Plasmid

    #133820

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    K326,327E

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX Syt1 C2AB (96-421) D303G F234C

    Plasmid

    #170647

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGEX Syt1 C2AB (96-421) D303G V304C

    Plasmid

    #170648

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G, C277A, V304C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGEX Syt1 C2AB (96-421) D365E F234C

    Plasmid

    #170650

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E, C277A, F234C

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge