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Ac 1.8kb red opsin GFP.

Plasmid

#72915

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Ac 1.8kb red opsin DsRed

Plasmid

#72914

Purpose

red opsin promoter from Carolina anole lizard (Anolis carolinensis) gDNA (nucleotides21,797 to 187, corresponding to chr2:88663108–88664997 in anoCar2.0) driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

red opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

Plasmid

#156414

Purpose

kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

EGFP

Expression

Mutation

mutant KRAS4B-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

Plasmid

#156415

Purpose

kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Zebrafish transgenesis

Tags

mTagBFP2

Expression

Mutation

mutant KRAS4A-G12D

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

Plasmid

#156416

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Zebrafish transgenesis

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

kdrl

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

Plasmid

#180468

Purpose

kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

KRAS4A (KRAS Human)

Use

Zebrafish transgenesis

Tags

V5-mScarlet-I

Expression

Mutation

Promoter

kdrl

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pQexp

Plasmid

#25820

Depositor

George Church

Article

Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Insert

pQexp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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His6-SpyCatcher-mi3

Plasmid

#113043

Purpose

Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

His6-SpyCatcher-mi3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Use

Tags

MGHHHHHHGGA

Expression

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Use

Tags

C-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQint

Plasmid

#25819

Depositor

George Church

Article

Tolonen et al Mol Microbiol. 2009 Dec . 74(6):1300-13.

Insert

qQint

Use

Bacterial gene inactivation

Tags

Expression

Mutation

plasmid to make targeted insertions in Clostridiu…

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pET21b-RL015A

Plasmid

#42134

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

sfGFP-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL027A

Plasmid

#42133

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCherry-mVenus

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET21b-RL020A

Plasmid

#42138

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mVenus-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pNoRBS

Plasmid

#218167

Purpose

pET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levels

Depositor

Emily Weinert

Article

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short insert

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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