We narrowed to 9,218 results for: Coli

1. pTETDuet_M42FG121VDHFR_R166QTS

   Plasmid

   #191298

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M42F/G121V DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrcECT

   Plasmid

   #75477

   Purpose

   Inducible expression of ectABC in E. coli

   Depositor

   Xixian Xie

   Article

   Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

   Insert

   ectABC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLA230

   Plasmid

   #11724

   Depositor

   Lawrence Loeb

   Article

   Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

   Insert

   b-lactamase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GAA for Glu26 changed to TAA (premature stop codo…

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. TGT-His

   Plasmid

   #138201

   Purpose

   Expresses E. Coli TGT with a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Insert

   E Coli tRNA Guanine Transglycosylase-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAM-RecG-ST2TaV-EGFP

   Plasmid

   #31275

   Depositor

   Andrew Pierce

   Article

   Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

   Insert

   RecG-ST2-TaV-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAM-RecG-EGFP

   Plasmid

   #31274

   Depositor

   Andrew Pierce

   Article

   Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

   Insert

   RecG-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. EcYbbN pET15b

   Plasmid

   #60690

   Purpose

   Recombinant protein expression of YbbN

   Depositor

   Mark Wilson

   Article

   Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

   Insert

   YbbN
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

9. MetNI (Lm)

   Plasmid

   #78316

   Purpose

   Methionine ABC Transporter

   Depositor

   Douglas Rees

   Article

   Kadaba et al Science. 2008 Jul 11;321(5886):250-3. do

   Inserts

   MetI
   MetN
   

   Use

   Tags

   10x Histidine Tag and 6x Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD33(A-N)

    Plasmid

    #187084

    Purpose

    Medium expression of Complex I with subunit N-HA tagged

    Depositor

    Steven Vik

    Article

    Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

    Insert

    nuoA-N
    

    Use

    Tags

    HA tag nuoN

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD33(EFG)

    Plasmid

    #187087

    Purpose

    Medium expression of subunits EFG

    Depositor

    Steven Vik

    Article

    Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

    Insert

    nuoEFG
    

    Use

    Tags

    HA tag nuoG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTETDuet_WTDHFR_WTTS

    Plasmid

    #191282

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT DHFR, WT TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW209

    Plasmid

    #113188

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAB172a

    Plasmid

    #156324

    Purpose

    Expresses heterologous rRNA

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    rRNA operon, orthogonal-antiRBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT antiRBS engineered to orthogonal antiRBS

    Promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSPK1

    Plasmid

    #18876

    Depositor

    Jay Keasling

    Article

    Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

    Insert

    polyphosphate kinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

16. PAS513 (TJF080)

    Plasmid

    #65580

    Purpose

    pETDuet-1 Containing FadD S379R

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S379R

    Promoter

    T7 lacO

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

17. PAS529 (TJF072)

    Plasmid

    #65603

    Purpose

    pETDuet-1 Containing FadD Q338R-His

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Q338R

    Promoter

    T7 lacO

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PAS504 (TJF067)

    Plasmid

    #65571

    Purpose

    pETDuet-1 Containing FadD F447S

    Depositor

    Pamela Silver

    Article

    Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

    Insert

    FadD with F447S mutation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F447S

    Promoter

    T7 lacO

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGU06

    Plasmid

    #212148

    Purpose

    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.

    Depositor

    Kang Zhou

    Insert

    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Budding Yeast, Agx1: S. cerevisiae; alaA, tyrB: E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTETDuet_F31YDHFR_R127ATS

    Plasmid

    #191312

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F31Y DHFR/R127A TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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