Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,400 results for: Coli

Showing: 401 - 420 of 9400 results

  2. pET30a-GABAT

    Plasmid
    #239572
    Purpose
    Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    4-aminobutyrate aminotransferase (gabT E. coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239572-plasmid-map-sequence-id-479014

    Enlarge

  3. pET30a-SSDH

    Plasmid
    #239573
    Purpose
    Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.
    Depositor
    William Plaxton
    Article
    Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b
    Insert
    Succinate-semialdehyde dehydrogenase (gabD E. coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239573-plasmid-map-sequence-id-476594

    Enlarge

  12. pmr101A-cPr-mCherry (KmR)

    Plasmid
    #177207
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177207-plasmid-map-sequence-id-348603

    Enlarge

  13. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

    Enlarge

  14. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

    Enlarge

  15. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

    Enlarge

  16. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

    Enlarge

  17. p416-GPD-PPK(mutant)

    Plasmid
    #183942
    Purpose
    Expresses catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli )
    Use
    Tags
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183942-plasmid-map-sequence-id-363372

    Enlarge

  18. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72399-plasmid-map-sequence-id-133993

    Enlarge

  19. pCASCADE-zwf

    Plasmid
    #65825
    Purpose
    zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    zwf guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available Since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65825-plasmid-map-sequence-id-124656

    Enlarge

  20. pCASCADE-udhA

    Plasmid
    #65818
    Purpose
    udhA targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available Since
    Aug. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    65818-plasmid-map-sequence-id-111976

    Enlarge

Showing: 401 - 420 of 9400 results