Addgene

Showing: 401 - 420 of 13586 results

Results per page:

Sort results by:

pcDNA3-MAVS-M1A500(KaganF60)

Plasmid

#52003

Purpose

Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A-500

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and deleted the C-terminal t…

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

Plasmid

#52018

Purpose

Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

Plasmid

#49062

Purpose

Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

Depositor

Biff Forbush

Insert

human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)

Use

Tags

3x Flag and mVenus

Expression

Mammalian

Mutation

N-terminal deletion of amino acids 13-222 in hNKC…

Promoter

CMV

Available since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin I (human)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP566

Plasmid

#222724

Purpose

pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH47742::p35S-ePEmax3::tEURb7

Plasmid

#227692

Purpose

Golden Gate level 1, position 2, ePEmax3 expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI820

Plasmid

#216629

Purpose

cpTurboID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

cpTurboID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI661 pRPR1(TetO)-sgRNA

Plasmid

#139475

Purpose

Vector for tet-regulated sgRNA expression in yeast

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Insert

single guide RNA scaffold

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pRPR1(TetO)

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pScPPX2

Plasmid

#112877

Purpose

Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

Depositor

Ursula Jakob, Michael Gray

Article

Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

Insert

PPX1 (PPX1 Budding Yeast)

Use

Tags

6X His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP1830

Plasmid

#222728

Purpose

pNP561 - T5 Target with T7 Inducible IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMH062

Plasmid

#225537

Purpose

Expresses IS621 in insect cells

Depositor

Hiroshi Nishimasu

Article

Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

Insert

IS621

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP555

Plasmid

#222722

Purpose

sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A, D731N

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge