We narrowed to 2,486 results for: organization Addgene

1. pcDNA5-FRT/TO-Flag-RNF169

   Plasmid

   #74243

   Purpose

   Expresses RNF169 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

   Insert

   RNF169 (RNF169 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pEF-pIgH-ESCargo(FTV,Crimson)

   Plasmid

   #140162

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSIIbsr-4082NES(ForAMPK)

   Plasmid

   #170310

   Purpose

   The lentiviral vector for AMPK FRET-biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

   Insert

   4082NES (FRET biosensor for AMPK activity)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-miniSOG-C1

   Plasmid

   #54801

   Purpose

   Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Type

   Empty backbone

   Use

   Tags

   mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pEF-pIgH-ESCargo*

   Plasmid

   #140159

   Purpose

   Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO APEX2-eIF4E1

   Plasmid

   #129644

   Purpose

   Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   EIF4E1 (EIF4E Human)
   

   Use

   Tags

   APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV6M-PAK1 H83L H86L K299R

   Plasmid

   #26592

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   H83L H86L K299R Dominant negative

   Promoter

   Available since

   March 9, 2011

   Availability

   Academic Institutions and Nonprofits only

9. px459 sgAtg5

   Plasmid

   #175023

   Purpose

   CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

   Depositor

   David Tumbarello

   Article

   Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

   Insert

   ATG5 (ATG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. miniSOG-RatLC3

    Plasmid

    #57775

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pSecTag-T210A-ATX

    Plasmid

    #17840

    Depositor

    Steven Rosen

    Article

    Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

    Insert

    T210A-ATX (ENPP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pEF-pIgH-ESCargo(FTV,Orange)

    Plasmid

    #140161

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUBC_V5_MS2-mVenus

    Plasmid

    #107606

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    MS2-mVenus
    

    Use

    Synthetic Biology

    Tags

    V5 and mVenus

    Expression

    Mutation

    MS2: V75E/A81G

    Promoter

    human UBC

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-miniSOG-N1

    Plasmid

    #54803

    Purpose

    Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pME-APEX2-NS

    Plasmid

    #67669

    Purpose

    Gateway entry clone containing APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKmyc-Borg3

    Plasmid

    #141074

    Purpose

    Expresses Borg3 (Cdc42ep5) in mammalian cells

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

    Insert

    Borg3 (Cdc42ep5 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUBC_HA_PP7-mCherry

    Plasmid

    #107605

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mCherry
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    hUBC

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. miniSOG-Rab5a-7

    Plasmid

    #57774

    Purpose

    Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Rab5a
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. K401-iLid

    Plasmid

    #122484

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Matt Thomson

    Article

    Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

    Insert

    Kinesin 1-401 iLid His tag
    

    Use

    Tags

    His6 and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-401

    Promoter

    T7

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-AKT-PH

    Plasmid

    #67674

    Purpose

    Gateway entry clone containing AKT-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge