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pCG_SARS-CoV1 NSP1 C-V5-tag

Plasmid

#179961

Purpose

Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

SARS-CoV-1 Nsp1 (ORF1ab Synthetic)

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCG_batCoV RaTG13 NSP15 C-V5-tag

Plasmid

#179984

Purpose

Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

Depositor

Konstantin Sparrer

Article

Hayn et al Cell Rep. 2021 May 18;35(7):109126.

Insert

RaTG13 Nsp15

Use

Tags

Expression

Mammalian

Mutation

human codon-optimized

Promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pET11a_3xFlag-nsp10 (SARS-CoV-2)

Plasmid

#169157

Purpose

To express SARS-CoV-2 nsp10 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-Strep-TEV-Nsp14

Plasmid

#154955

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)

Use

Tags

Strep-TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pLV.SP146.gp91.GP91.cHS4

Plasmid

#30480

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

SP146/gp91/GP91/cHS4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-nsp13 (SARS-CoV-2)

Plasmid

#169190

Purpose

Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

GST

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1 R152Q GFP

Plasmid

#51884

Purpose

expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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7093 pNOTAG Hsp104 T317A

Plasmid

#1242

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (T317A) (HSP104 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

T317A

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pET28-Nsp14-TEV-Strep

Plasmid

#154953

Purpose

Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET22-Strep-TEV-Nsp10

Plasmid

#154958

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

Depositor

Justin Kollman

Insert

Strep-TEV-Nsp10 (ORF1ab SARS-CoV-2)

Use

Tags

Strep-TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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7090 pNOTAG Hsp104 N728A

Plasmid

#1244

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (N728A) (HSP104 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

N728A

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
7092 pNOTAG Hsp104 R826M

Plasmid

#1243

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (R826M) (HSP104 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

R826M

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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