Addgene

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Plxna4-AP-His

Plasmid

#71999

Purpose

Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna4 (Plxna4 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

Plasmid

#125148

Purpose

Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

AcrIIA4-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-AP-His

Plasmid

#72019

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hcMYC (Plath)

Plasmid

#17966

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

v-myc myelocytomatosis viral oncogene homolog (avian) (MYC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pMP71-mIL15-IL12rb1-sushi-il12rb2

Plasmid

#174606

Purpose

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Neo1.a-AP-His

Plasmid

#71963

Purpose

Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.a (Neo1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-hKLF4 (Plath)

Plasmid

#17967

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Kruppel-like factor 4 (gut) (KLF4 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sema6c-Fc-His

Plasmid

#72167

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-mJP-4

Plasmid

#79598

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Woo et al Proc Natl Acad Sci U S A. 2016 Mar 8;113

Insert

Junctophilin-4 (Jph4 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-hOCT4 (Plath)

Plasmid

#17964

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

POU domain, class 5, transcription factor 1 (POU5F1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pMXs-hSOX2 (Plath)

Plasmid

#17965

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

SRY (sex determining region Y)-box 2 (SOX2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Sema3e(L)-Fc-His

Plasmid

#72145

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6d.1-AP-His

Plasmid

#72042

Purpose

Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.1 (Sema6d Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
Sema4a-Fc-His

Plasmid

#72154

Purpose

Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4a (Sema4a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

Plasmid

#203439

Purpose

Mammalian expression of RUNX4

Depositor

Yoshiaki Ito

Article

Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

Insert

RUNX3 (RUNX3 Human)

Use

Tags

3 x FLAG

Expression

Mammalian

Mutation

R122C

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Sema6d.1-Fc-His

Plasmid

#72168

Purpose

Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.1 (Sema6d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-R175H-N268D

Plasmid

#35086

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R175H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only