162,160 results

1. pFA6a-6xGLY-FLAG-hphMX4

   Plasmid

   #20752

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-RO1.7-RPE65

   Plasmid

   #206346

   Purpose

   AAV plasmid expressing RPE65 in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

   Insert

   Rpe65 (Rpe65 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   450-Met (C57BL6 natural variation)

   Promoter

   1.7kb human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSV-CaMKII(1-290)

   Plasmid

   #45065

   Purpose

   Expression of constit active CaMKII

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   CaM Kinase II (Camk2a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   truncated at amino acid 290

   Promoter

   RSV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK642

   Plasmid

   #72391

   Purpose

   Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-Flag-hS1pr1_Neo

   Plasmid

   #203709

   Purpose

   Overexpression of FLAG-S1PR1.

   Depositor

   Timothy Hla

   Insert

   Flag-hS1PR1 (S1PR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0915

   Plasmid

   #143451

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NCOA2 (NCOA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST51-LRRK2-R1441C

   Plasmid

   #25081

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Destination vector

   Tags

   6XHis and V5

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pLV Rnf121-V5

   Plasmid

   #175130

   Purpose

   Lentiviral expression of mouse Rnf121-V5

   Depositor

   Larry Gerace

   Insert

   Rnf121 (Rnf121 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB418

   Plasmid

   #74812

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRKH3-ermGFP

    Plasmid

    #27169

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    presence of an additional 183-bp region at N term…

    Promoter

    Available since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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