We narrowed to 12,598 results for: 112
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Plasmid#213384PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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VIPR2-DuET
Plasmid#213387PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGDR-DuET
Plasmid#213369PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-Prf1-ires-mCherry
Plasmid#209073PurposeTargeting vector for the mouse Prf1 locus to correct prf1 geneDepositorInsertPrf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
UseCRISPRAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-PRF1-T2A-cDNA
Plasmid#209076PurposeTargeting vector for the human PRF1 locus to replace exon 3 with repaired cDNADepositorInsertPRF1-T2A-cDNA HDRT (PRF1 Human)
UseCRISPRAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPPromoterelavl3(HuC)Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
Plasmid#194698PurposeHuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_off-mEGFP
UseZebrafish expressionTagsmEGFPPromoterelavl3(HuC)Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-IG-ATG3 (K62D/K64D)
Plasmid#212031PurposeExpresses untagged ATG3 (K62D/K64D) in mammalian cellsDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-COMMD3_CDS-V5-hygro
Plasmid#195509Purposelentiviral vector expressing COMMD3 CDS with hygromycin selectionDepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-Δ220-234-Flag
Plasmid#210341PurposeExpresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationΔ220-234Available SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-H120N/F121L-Flag
Plasmid#210340PurposeExpresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationH120N/F121LAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-FR-2Strep
Plasmid#210316PurposeExpresses SARS-CoV-2 NSP4 in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
Tags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-mCherry
Plasmid#210319PurposeExpresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsmCherryExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
STIM1-2Strep
Plasmid#210361PurposeExpresses STIM1 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP10-2Strep
Plasmid#210357PurposeExpresses FKBP10 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNAJC11-2Strep
Plasmid#210354PurposeExpresses DNAJC11 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ZNF746-C571A
Plasmid#207821PurposeN-terminal tagged ZNF746-C571A for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-ZNF746-C571A (ZNF746 Human)
UseLentiviralTagsminiTurbo-V5MutationC571APromoterUBCAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-ZNF746
Plasmid#207822PurposeDual expression of Cas9 and sgRNA targeting ZNF746DepositorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCOA-Leu-FvPPT1
Plasmid#179923PurposeExpression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertFvPPT1
ExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A)
Plasmid#208088PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.DepositorAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(S310A)
Plasmid#208089PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.DepositorAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_LTB4R(T308A,S310A)
Plasmid#208090PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.DepositorInsertLTB4R(T308A,S310A) (LTB4R Human)
UseLentiviralExpressionMammalianMutationT308A, S310APromoterPGKAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R
Plasmid#208091PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationnonePromoterPGKAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB4
Plasmid#200983PurposeMammalian expression plasmid for myc-tagged EphB4DepositorInsertEphB4 (EPHB4 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
Tags6xHis-tagExpressionBacterial, Insect, and Mamm…Promoterpolh, CAG, T7Available SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-H262A
Plasmid#202782PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stopDepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-T244A
Plasmid#202781PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stopDepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-Y209A
Plasmid#202779PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stopDepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-Y210A
Plasmid#202780PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stopDepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-K208D
Plasmid#202785PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stopDepositorAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-C264A
Plasmid#202778PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stopDepositorAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-R265A
Plasmid#202783PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stopDepositorAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-ATG3-H266A
Plasmid#202784PurposeATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stopDepositorAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-NeoCOVGT3-d2EGFP-mCherry
Plasmid#201450PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT3_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP-mCherry
Plasmid#201451PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP-mCherry
Plasmid#201452PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT4-d2EGFP-mCherry
Plasmid#201453PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT4_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IGFBP2-Clover
Plasmid#191007PurposeGateway entry vector with IGFBP2 tagged with CloverDepositorInsertInsulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
UseGateway entry vectorTagsCloverAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-NDUFB5_sgRNA1
Plasmid#201606PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertNDUFB5 (NDUFB5 Human)
UseCRISPR and LentiviralAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-NDUFB5_sgRNA2
Plasmid#201607PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertNDUFB5 (NDUFB5 Human)
UseCRISPR and LentiviralAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ control-FLAG
Plasmid#199678PurposeBacterial expression of Leucine Zipper (LZ) control-FlagDepositorInsertLeucine zipper control
Tags6xHis and FlagExpressionBacterialPromoterT7 promoterAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-Cterm-V5His6_H
Plasmid#146488PurposeInsect Expression of DmPABPC1-CtermDepositorInsertDmPABPC1-Cterm (pAbp Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-Nterm-V5His6_H
Plasmid#146489PurposeInsect Expression of DmPABPC1-NtermDepositorInsertDmPABPC1-Nterm (pAbp Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-DmPABPC1-Nterm-V5His6_H
Plasmid#146478PurposeInsect Expression of DmPABPC1-NtermDepositorInsertDmPABPC1-Nterm (pAbp Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G
Plasmid#146388PurposeInsect Expression of DmGW182_1-830-dsRNAresDepositorInsertDmGW182_1-830-dsRNAres (gw Fly)
ExpressionInsectMutationtwo non silent mutations V704A and N799S, and one…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182_1-1115-dsRNAres_G
Plasmid#146389PurposeInsect Expression of DmGW182_1-1115-dsRNAresDepositorInsertDmGW182_1-1115-dsRNAres (gw Fly)
ExpressionInsectMutationtwo non silent mutations V704A and N799S, and one…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S
Plasmid#147461PurposeMammalian Expression of HsSmg7-G100E-siRNAres-shRNAresDepositorInsertHsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S
Plasmid#147462PurposeMammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAresDepositorInsertHsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-T103E-siRNAres_S
Plasmid#147464PurposeMammalian Expression of HsSmg7-T103E-siRNAres-shRNAresDepositorInsertHsSmg7-T103E-siRNAres-shRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only