We narrowed to 4,564 results for: HRE

1. pYI2206

   Plasmid

   #228348

   Purpose

   Constitutive rPhlTA mutant expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMT1026

   Plasmid

   #228299

   Purpose

   Constitutive expression of PhlTA mutant with weak promoter and terminator

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   E41G

   Promoter

   KpYPT1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMT478

   Plasmid

   #228286

   Purpose

   Constitutive mutant rPhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   rphlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-parkin T103A

   Plasmid

   #227360

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin T103A (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. psiCHECK-1-miR-376a1(5p)-WT

   Plasmid

   #215875

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. psiCHECK-1-miR-376a1(3p)-WT

   Plasmid

   #215876

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. psiCHECK-1-miR-589(5p)-WT

   Plasmid

   #215877

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-WT (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK-1-miR-589(3p)-WT

   Plasmid

   #215878

   Purpose

   This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

   Insert

   three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK-1-miR-376a2(3p)-WT

    Plasmid

    #215879

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK-1-miR-99a-WT

    Plasmid

    #215880

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiCHECK-1-miR-376a1(5p)-I-type

    Plasmid

    #215882

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psiCHECK-1-miR-22(3p)-I-type

    Plasmid

    #215886

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psiCHECK-1-miR-376a2(3p)-I-type

    Plasmid

    #215887

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psiCHECK-1-miR-99a-I-type

    Plasmid

    #215888

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC3_C_CCDC22(T17A)

    Plasmid

    #209694

    Purpose

    Expresses MAC3-tagged CCDC22(T17A) in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

    Insert

    CCDC22 (CCDC22 Human)
    

    Use

    Tags

    MAC3-tag

    Expression

    Mammalian

    Mutation

    T17A, changed Threonine 17 to Alanine

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. T40A

    Plasmid

    #217911

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET151-GyrB_T522

    Plasmid

    #216235

    Purpose

    S. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Changed threonine 522 to isoleucine

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCL1318

    Plasmid

    #217960

    Purpose

    Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H4 (H4C1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    L63A, F66A, I71A

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FB400

    Plasmid

    #203636

    Purpose

    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    FB367+FB397
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge