We narrowed to 4,634 results for: HRE

1. AAV-FLEX-FLIM-AKART391A

   Plasmid

   #60446

   Purpose

   Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   FLIM-AKART391A
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 391 to Ananine

   Promoter

   CAG

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 Flag MKK3b(Glu)

   Plasmid

   #50449

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Serine 218 to Glutamic Acid, changed Thre…

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. 3341 pcDNA3 Bcl-2 T69A S70A S87A

   Plasmid

   #13340

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 69 to Alanine; changed Serine 7…

   Promoter

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159850

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR P2R-P3-mKate2

   Plasmid

   #48345

   Purpose

   Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   mKate2
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a stop codon at the 3' end of the c…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P2R-P3-ECFP

   Plasmid

   #48353

   Purpose

   Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   ECFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR P4-P1R-EYFP

   Plasmid

   #48350

   Purpose

   Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159854

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiV_Neo_Sik3_T142Q

   Plasmid

   #119973

   Purpose

   Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Insert

   Sik3 (Sik3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   change Threonine 142 to Glutamine

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191155

    Purpose

    Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:DNAJB1.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMyc4ElbLuc.

    Plasmid

    #53246

    Purpose

    reporter plasmid containing 4 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc4ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5 Flag-Smad1 (AAVA)

    Plasmid

    #14957

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

    Insert

    Smad1 (AAVA) (SMAD1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Three Serine to Alanine mutations in the C-termin…

    Promoter

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

13. MSP3-bio

    Plasmid

    #47731

    Purpose

    Expresses enzymatically monobiotinylated full-length MSP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised MSP3
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH727-CEN-RLuc/staCFLuc

    Plasmid

    #38211

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-RIPK3[S227D]-FLAG

    Plasmid

    #199320

    Purpose

    mutated phosphorylation site (MLKL interaction permanent active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Aspartic Acid

    Promoter

    Available Since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-RIPK3[S227A]-FLAG

    Plasmid

    #199318

    Purpose

    mutated phosphorylation site (MLKL interaction inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Alanine

    Promoter

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159858

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMyc3ElbLuc

    Plasmid

    #53245

    Purpose

    reporter plasmid containing 3 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc3ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCFJ2438 - [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159862

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mCardinal((PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IP-EGFP-mouse Plaat3 (N157stop)

    Plasmid

    #168771

    Purpose

    Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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