We narrowed to 10,563 results for: CHL

1. pBABE-hygro-BAP1-G312G-HA

   Plasmid

   #154022

   Purpose

   Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G

   Depositor

   Samuel Peña-Llopis

   Article

   Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   c.936T>G, p.G312G (Synonymous Mutation) by Sit…

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdCas9-M-C4

   Plasmid

   #73427

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. shCCL20

   Plasmid

   #65096

   Purpose

   retroviral expression of CCL20 shRNA

   Depositor

   Pau Sancho-Bru

   Article

   Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

   Insert

   Ccl20 shRNA (Ccl20 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-NES-YFP-CCTd

   Plasmid

   #184326

   Purpose

   Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.3)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTd (CACNA1D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-NLS-YFP-CCTd

   Plasmid

   #184325

   Purpose

   Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTd (CACNA1D Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pdCas9-M-3F2

   Plasmid

   #73428

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEW108

   Plasmid

   #232823

   Purpose

   Expresses yeast compass complex in insect cells

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

   Insert

   COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
   

   Use

   Tags

   His6-3xFLAG, Twinstrep

   Expression

   Insect

   Mutation

   Set1(762-1080)

   Promoter

   polyhedrin promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

   Plasmid

   #195048

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pADH2, pGPD, and pXYL

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

   Plasmid

   #195047

   Purpose

   cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Inserts

   crtE
   crtI
   crtYB
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pADH2, pGPD, and pXYL

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ku70 insUP+ pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD-crtI-tNOS-pADH2-crtYB-tNOS+ku70 insD (SBE144)

    Plasmid

    #195046

    Purpose

    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Inserts

    crtE
    crtI
    crtYB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pADH2, pGPD, and pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMMACE DEST CMV PE2

    Plasmid

    #206276

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes PE2 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    PE2
    

    Use

    CRISPR; Recombinant baculovirus production, multi…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-NLS-YFP-CCTc

    Plasmid

    #184327

    Purpose

    Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-ccdB-boxB-tBE-V5-mA3

    Plasmid

    #171693

    Purpose

    Expresses tBE-V5-mA3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1a

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. RG6-BAP1_E11

    Plasmid

    #154025

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. RG6-BAP1_E7

    Plasmid

    #154023

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pdCas9-M-4F2

    Plasmid

    #73431

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 4F2 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdCas9-M-1B6

    Plasmid

    #73430

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1B6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pdCas9-M-3H5

    Plasmid

    #73429

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 3H5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pdCas9-M-1D4

    Plasmid

    #73433

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1D4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pdCas9-M-1E4

    Plasmid

    #73436

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 1E4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pdCas9-M-5F5

    Plasmid

    #73432

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor 5F5 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX304-GW-DCK*-IRES-GFP

    Plasmid

    #176291

    Purpose

    Gateway vector for use in generating POI-DCK* fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plenti-FLAG-CAMK2D

    Plasmid

    #221699

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. plenti-FLAG-CAMK2D-ED

    Plasmid

    #221700

    Purpose

    Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K43R, D136N

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-bARGSer-AxeTxe

    Plasmid

    #192473

    Purpose

    Second-generation bacterial acoustic reporter gene derived from Serratia

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Inserts

    bARGSer
    AxeTxe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    the gene Ser39006_001280 was deleted from the clu…

    Promoter

    Native promoter from Enterococcus faecium and pBAD

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65454

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid

    #127090

    Purpose

    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

    Depositor

    Viviana Gradinaru

    Article

    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

    Has Service

    AAV PHP.eB

    Insert

    Channelrhodopsin-2
    

    Use

    AAV

    Tags

    eYFP (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Humanized ChR2 gene with histidine 134 changed to…

    Promoter

    CAG

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX304-DCK*-GW-IRES-GFP

    Plasmid

    #176289

    Purpose

    Gateway vector for use in generating DCK*-POI fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOTTC468 - pAAV EF1a DIO hChR2(H134R)-iRFP

    Plasmid

    #47633

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2 (H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223_BAP1_WT

    Plasmid

    #81852

    Purpose

    Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Spike Display_Part 2 Spacer

    Plasmid

    #172730

    Purpose

    Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid

    #169185

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV phSyn1-RSR-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid

    #65429

    Purpose

    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Dre and Cre recombinases

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    H134R variant

    Promoter

    hSyn1

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201820

    Purpose

    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169184

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169183

    Purpose

    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag (nsp12 C-terminus) and His6 (as internal t…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Anti-Cavbeta1 Ca2+ channel [N7/18.1R]

    Plasmid

    #114497

    Purpose

    Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18.1.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Cavbeta1 Ca2+ channel (Home sapiens) recombinant mouse monoclonal antibody (CACNB1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDONR223_BAP1_p.N299S

    Plasmid

    #81572

    Purpose

    Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    N299S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDONR223_BAP1_p.D75G

    Plasmid

    #81526

    Purpose

    Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D75G

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Cavbeta1 Ca2+ channel scFv [N7/18]

    Plasmid

    #206789

    Purpose

    Mammalian Expression of Cavbeta1 Ca2+ channel scFV. Derived from hybridoma N7/18 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Cavbeta1 Ca2+ channel (Homo sapiens) recombinant scFV (CACNB1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201821

    Purpose

    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid

    #201822

    Purpose

    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    hChR2(H134R)-V5-ER-LBD (ChRERa)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKii

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Spike Display_Part 5 Spacer

    Plasmid

    #172733

    Purpose

    Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. Spike Display_Part 4 Spacer

    Plasmid

    #172732

    Purpose

    Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. Spike Display_Part 3 Spacer

    Plasmid

    #172731

    Purpose

    Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

50. Spike Display_Part 1 Spacer

    Plasmid

    #172729

    Purpose

    Encodes Part 1 of HexaPro-D614G spike to be used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge