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pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

Plasmid

#228722

Purpose

Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2702-CMV-iNme2-ABE8e-nt

Plasmid

#228723

Purpose

Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2 nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2703-CMV-iNme2Smu-ABE8e-nt

Plasmid

#228724

Purpose

Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

N-terminal ABE8e with iNme2Smu nCas9 (D16A)

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgMyc_2

Plasmid

#138346

Purpose

Express guide RNA 1 for mouse Myc

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

cMyc sgRNA (Myc Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3015b

Plasmid

#209317

Purpose

Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

Depositor

Thomas Hooven

Article

Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

Insert

Group B Streptococcus-compatible single guide RNA

Expression

Bacterial

Promoter

Xyl/tet promoter that is constitutively active

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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SWEET11:SWEET11-2A-GFP-GUS

Plasmid

#172484

Purpose

Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

Depositor

Wolf Frommer

Article

Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

Insert

SWEET11 (AT3G48740 Mustard Weed)

Tags

GFP, GUS

Expression

Plant

Promoter

SWEET11

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL1M-pMtRC-MtLAP1-Adh-R1

Plasmid

#170790

Purpose

A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

Depositor

Jeremy Murray

Article

Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

Insert

MtLAP1 (LOC11412013 Medicago truncatula)

Expression

Bacterial and Plant

Promoter

Medtr4g059670(MtRC)

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0762 (pQCascade_crRNA-nt)

Plasmid

#130636

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)

Use

CRISPR; Transposon

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Expression

Insect

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3a-T1

Plasmid

#41821

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T1 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pUB-nHyPer2 (NLS:HyPer2:NLS)

Plasmid

#84730

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS (nuclear localisation signal and NLS (nuclear…

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3a-T2

Plasmid

#41822

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T2 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

Plasmid

#200910

Purpose

3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

Depositor

Debojyoti Chakraborty

Insert

3XHA_den1FnCas9_KRAB_eGFP

Use

CRISPR

Tags

3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

Expression

Mammalian

Mutation

Changed Glutamic Acid to Arginine at 1369 position

Promoter

CBH

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3b

Plasmid

#41823

Purpose

Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3b (DNMT3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX Myc-HA

Plasmid

#229499

Purpose

For lentiviral expression of Myc coding sequence with an HA tag.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

MYC

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS2603_AAV-Nme2-ABE8e-i1-U6-Rosa26

Plasmid

#201685

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 driven sgRNA targeting the mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA (GB0645)

Plasmid

#68210

Purpose

Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SgRNA

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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