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pcDNA3.1-SARS-Spike
Plasmid#145031PurposeExpress SARS-CoV spike protein with C9 tag at C-terminal in mammalian cellsDepositorInsertSARS-CoV Spike
UseTagsC9 and CD5 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRB_SCF2_LD6_CTEV
Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
2MLS-HyperNova/pcDNA3
Plasmid#221457PurposeExpressing HyperNova in mitochondriaDepositorInsertHyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP_NR2B
Plasmid#23998DepositorInsertNR2B (Grin2b Rat)
UseTagsNR2B signal sequence (aa1-31) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-Ecadherin
Plasmid#65286Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOT_7-lenti-EFS-SS1-BBz-2A-puro
Plasmid#217990PurposeExpresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.DepositorInsertSS1-BBz CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-tdTomato-cNLS
Plasmid#138529PurposeInducible expression of tdTomato with nuclear localization signalDepositorInserttdTomato
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-NES-ZapCV2-Zinc dead
Plasmid#203850PurposeExpressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsNuclear Export SignalExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEHlyA5
Plasmid#168298PurposeSecretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyADepositorInsertN‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
UseTagsE-tag, HA-tag, His-tag, Prescission site, and VHH…ExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-GCaMP6m-Xn
Plasmid#118976PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleusDepositorInsertGCaMP6m-XN
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-Ecadherin
Plasmid#65287Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2
Plasmid#84603PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsODC1 destabilizing elementExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9 Null
Plasmid#198727PurposeExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.DepositorInsertCas9
UseTagsExpressionPlantMutationPromoterpG10-90Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCoofy57
Plasmid#122009PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His, PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis8 and Multi1: depending on the LP2 primer, eit…ExpressionMammalianMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiAsCpf1-3xMYC-blast
Plasmid#127750PurposeLentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signalDepositorInsertAsCpf1
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCX-SponGee
Plasmid#134775Purposemammalian expression of SponGee, a buffer of cGMP that prevents downstream signalingDepositorInsertSponGee
UseTagsFLAG and mRFPExpressionMammalianMutationPromoterCAGAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP
Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-2X snRP1
Plasmid#99688PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
UseTagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only
