We narrowed to 10,106 results for: HA
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pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCH61 (multiAsCas12a-KRAB piggyBac)
Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA
Plasmid#200641PurposeExpresses miniTurboID-FKBPF36V-2xHA in mammalian cells.DepositorInsertminiTurboID-6xGGSG linker-FKBPF36V-2xHA
UseTagsHA-tagExpressionMammalianMutationPromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZW6 (CK2alpha)
Plasmid#27086DepositorInsertCK2alpha (CSNK2A1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUHD.NFATc1nuc
Plasmid#40616DepositorInsertNFATc1nuc (NFATC1 Human)
UseTagsHAExpressionMammalianMutationS172A S175A S176A S178A S179A S181A S184A S187A S…PromoterCMV2Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pZW16 (CK2alpha')
Plasmid#27087DepositorInsertCK2alpha' (CSNK2A2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertYFP-SMASh(AI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-YFP-SMASh(TI)
Plasmid#111501PurposeExpresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].DepositorInsertYFP-SMASh(TI)
UseTagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAHA
Plasmid#44169DepositorInsertshemagglutinin gene
neuraminidase gene
UseTagsExpressionMammalianMutationT55APromoterCMV enhancer/human Ferritin light chain promoter …Available sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2w/b
Plasmid#47586DepositorInsertPMCA2w/b (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NS3-NLS/VPR
Plasmid#112244PurposeEncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.DepositorInsertdCas9-NS3-NLS/VPR
UseTagsAU1 (N term on NS3) and HA (C term on NS3)ExpressionMammalianMutationNS3 mutant T54APromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1211YN
Plasmid#61704PurposeGateway compatible BiFC vector (pEarleyGate201-YN)DepositorTypeEmpty backboneUseBifc assayTagsYFP-N and HAExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Geminin-mStrawberry reporter
Plasmid#158690PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
Plasmid#219782PurposeCaST-IRES for HEK cell expressionDepositorInsertCD4-sTC-M13-GFP-IRES-CaM-V5-sTN
UseAAVTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
013LP-pAAV-ANF-657-EmGFP-pA
Plasmid#120267PurposeAAV vector for Atrial specific EmGFP expressionDepositorInsertEmGFP
UseAAVTags3XFlag and HAExpressionMutationPromoterANF promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed-Rab5 DN
Plasmid#13051DepositorInsertRAB5A (Canis familiaris) (RAB5A Canis familiaris)
UseTagsDsRed2 and HAExpressionMammalianMutationasparagine 3 changed to serine (neutral); aspara…PromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-8his
Plasmid#111847PurposeMammalian expression plasmid for soluble BG505 SOSIP.664DepositorInsertHIV-1 Env (BG505 SOSIP.664)
UseTags8his purification tag and CD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; has SOSIP mutatio…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
Plasmid#219781PurposeCaST-P2A for HEK cell expressionDepositorInsertCD4-sTC-M13-GFP-P2A-CaM-V5-sTN
UseAAVTagsHA and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
UseTags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianMutationPromoterhUbCAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6610 MSCV-IP N-HAonly TIMP1
Plasmid#35009DepositorInsertTIMP1 (TIMP1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-Bmi1
Plasmid#31783DepositorInsertBmi1 (BMI1 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-GFE3
Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZRS-hNEDD9_IresGFP
Plasmid#21962DepositorInsertNEDD9 (NEDD9 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-LID
Plasmid#31767DepositorInsertYFP-LID (FKBP1A Human)
UseRetroviralTagsHA and YFPExpressionMutationF36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…PromoterLTRAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAExpressionMutationPromotergfaABC1D (astrocyte specific)Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only
