We narrowed to 6,668 results for: PROC

1. pENTR1A mKate2-hu_ncOGT K842M

   Plasmid

   #154284

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   K842M mutation (Catalytic Inactive)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

   Plasmid

   #58492

   Purpose

   retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

   Depositor

   David Ron

   Article

   Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

   Insert

   IRE1a (ERN1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and preprotrypsin signal sequence

   Expression

   Mammalian

   Mutation

   deleted amino acids P29 to D408 and bearing mutat…

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR-Flag-IKKbeta-KM

   Plasmid

   #15466

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta-KM (Ikbkb Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Available Since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

4. J-sRNA-331Bb

   Plasmid

   #78285

   Purpose

   An inducible small RNA with E.coli Hfq targeting K. rhaeticus UGPase mRNA

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   j-sRNA-331
   

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR-HA-IKKalpha

   Plasmid

   #15469

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha (Chuk Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

6. CALID

   Plasmid

   #169708

   Purpose

   Cre-activated light-inducible Dre recombinase

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 MLK1 E179K

   Plasmid

   #60541

   Purpose

   Donor vector for human MLK1 E179K mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Mutation

   E179K

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Mouse Alpha2 delta-3 pMT2

   Plasmid

   #58727

   Purpose

   expression of mouse Alpha2 delta-3 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

   Insert

   alpha2 delta-3 (Cacna2d3 Mouse)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-PSAT

   Plasmid

   #74115

   Purpose

   Expression of PSAT

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   pET28a-PSAT (PSAT1 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 MLK1 D294A

    Plasmid

    #60542

    Purpose

    Donor vector for human MLK1 D294A mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Mutation

    D294A

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pPL001

    Plasmid

    #184750

    Purpose

    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    pEF1a-eGFP-T2A-BSD-bGHpA
    

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q

    Plasmid

    #60420

    Purpose

    Mammalian Expression of ITSN DH/PH-tgRFPt-Micro

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    hITSN 1159-1509 // SSPB R73Q, Y11K and A15E

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. SunTag-FWAgRNA-4-14aa-TET1cd

    Plasmid

    #106436

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Human, Synthetic, Mustard Weed)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLP-PIGA2 (pLP300)

    Plasmid

    #168462

    Purpose

    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

    Depositor

    Matthew Maurano

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Type

    Empty backbone

    Use

    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-PHD2-181-426

    Plasmid

    #223551

    Purpose

    His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-180.

    Promoter

    trc

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Best1-sCX3CL1

    Plasmid

    #164422

    Purpose

    AAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    Cx3cl1 (Cx3cl1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    deleted terminal 59 amino acids

    Promoter

    Best1

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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