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lentiCRISPRv2_sgCtrl#1

Plasmid

#174148

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLG028 (hhe)

Plasmid

#157906

Purpose

Expresses the hhe defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

hhe (DUF4011-helicase-Vsr-DUF3320)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4

Plasmid

#62899

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRF-BetI

Plasmid

#49357

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

BetI repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4-AMBRA1-3xFLAG

Plasmid

#174157

Purpose

Vector for expression of 3xFLAG-tagged wild-type AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMLS7

Plasmid

#32056

Purpose

Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Expression

Bacterial

Promoter

Ps7 promoter, originated from Paraburkholderia xe…

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCRE-iRFP670

Plasmid

#82696

Purpose

Expresses CRE and iRFP670 under two separate PGK promoters.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Insert

CRE, iRFP670

Use

Cre/Lox

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAD

Plasmid

#64747

Purpose

Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser659ala, ser1157ala)

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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PGL3Basic-Mdm2-T1

Plasmid

#32365

Depositor

Hong Wu

Article

Chang et al J Biol Chem. 2004 Jul 9;279(28):29841-8.

Insert

Mdm2 promoter (Mdm2 Mouse)

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF1-V2

Plasmid

#69045

Purpose

expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

splice variant 2

Promoter

IRES

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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p405MET25

Plasmid

#15961

Depositor

Nicolas Buchler, Fred Cross

Insert

MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

MET25 promoter is between SacI and XbaI restricti…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-LSL-nlsGFP-shRNA

Plasmid

#175584

Purpose

Conditional express shRNA with control of LoxP

Depositor

Christian Wolfrum

Article

Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

Type

Empty backbone

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EFS

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-mMcl1

Plasmid

#25714

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1 (Mcl1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pHDR-CDKN2A-Ex2-CMV-EGFP

Plasmid

#110734

Purpose

Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

EGFP

Use

Other

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Mutation

see attached file

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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